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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleInhibition Of Fatty Acid Synthase In Melanoma Cells Activates The Intrinsic Pathway Of Apoptosis.pt_BR
dc.contributor.authorZecchin, Karina Gpt_BR
dc.contributor.authorRossato, Franco Apt_BR
dc.contributor.authorRaposo, Helena Fpt_BR
dc.contributor.authorMelo, Daniela Rpt_BR
dc.contributor.authorAlberici, Luciane Cpt_BR
dc.contributor.authorOliveira, Helena C Fpt_BR
dc.contributor.authorCastilho, Roger Fpt_BR
dc.contributor.authorColetta, Ricardo Dpt_BR
dc.contributor.authorVercesi, Aníbal Ept_BR
dc.contributor.authorGraner, Edgardpt_BR
unicamp.authorKarina G Zecchin, Departamento de Diagnóstico Oral, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, Piracicaba, São Paulo, Brazil.pt_BR
unicamp.author.externalFranco A Rossato,pt
unicamp.author.externalHelena F Raposo,pt
unicamp.author.externalDaniela R Melo,pt
unicamp.author.externalLuciane C Alberici,pt
unicamp.author.externalHelena C F Oliveira,pt
unicamp.author.externalRoger F Castilho,pt
unicamp.author.externalRicardo D Coletta,pt
unicamp.author.externalAníbal E Vercesi,pt
unicamp.author.externalEdgard Graner,pt
dc.subjectAnalysis Of Variancept_BR
dc.subjectAnimalspt_BR
dc.subjectApoptosispt_BR
dc.subjectCalciumpt_BR
dc.subjectCaspasespt_BR
dc.subjectCell Cyclept_BR
dc.subjectCell Line, Tumorpt_BR
dc.subjectCell Proliferationpt_BR
dc.subjectCell Survivalpt_BR
dc.subjectCeruleninpt_BR
dc.subjectCytochromes Cpt_BR
dc.subjectDna Primerspt_BR
dc.subjectEnzyme-linked Immunosorbent Assaypt_BR
dc.subjectFatty Acid Synthasespt_BR
dc.subjectFatty Acid Synthesis Inhibitorspt_BR
dc.subjectFlow Cytometrypt_BR
dc.subjectLactonespt_BR
dc.subjectLipidspt_BR
dc.subjectMelanomapt_BR
dc.subjectMicept_BR
dc.subjectRna Interferencept_BR
dc.subjectReactive Oxygen Speciespt_BR
dc.description.abstractFatty acid synthase (FASN) is the metabolic enzyme responsible for the endogenous synthesis of the saturated long-chain fatty acid, palmitate. In contrast to most normal cells, FASN is overexpressed in a variety of human cancers, including cutaneous melanoma, in which its levels of expression are associated with tumor invasion and poor prognosis. We have previously shown that FASN inhibition with orlistat significantly reduces the number of spontaneous mediastinal lymph node metastases following the implantation of B16-F10 mouse melanoma cells in the peritoneal cavity of C57BL/6 mice. In this study, we investigate the biological mechanisms responsible for the FASN inhibition-induced apoptosis in B16-F10 cells. Both FASN inhibitors, cerulenin and orlistat, significantly reduced melanoma cell proliferation and activated the intrinsic pathway of apoptosis, as demonstrated by the cytochrome c release and caspase-9 and -3 activation. Further, apoptosis was preceded by an increase in both reactive oxygen species production and cytosolic calcium concentrations and independent of p53 activation and mitochondrial permeability transition. Taken together, these findings demonstrate the mitochondrial involvement in FASN inhibition-induced apoptosis in melanoma cells.en
dc.relation.ispartofLaboratory Investigation; A Journal Of Technical Methods And Pathologypt_BR
dc.relation.ispartofabbreviationLab. Invest.pt_BR
dc.date.issued2011-Febpt_BR
dc.identifier.citationLaboratory Investigation; A Journal Of Technical Methods And Pathology. v. 91, n. 2, p. 232-40, 2011-Feb.pt_BR
dc.language.isoengpt_BR
dc.description.volume91pt_BR
dc.description.firstpage232-40pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1530-0307pt_BR
dc.identifier.doi10.1038/labinvest.2010.157pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/20805790pt_BR
dc.date.available2015-11-27T13:21:24Z-
dc.date.accessioned2015-11-27T13:21:24Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:21:24Z (GMT). No. of bitstreams: 1 pmed_20805790.pdf: 349634 bytes, checksum: ab58d8cc1a7da6844d248618f8a7bfc1 (MD5) Previous issue date: 2011en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/199421-
dc.identifier.idPubmed20805790pt_BR
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