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Type: Artigo de periódico
Title: Evolution On Experimental Animal Model For Upper Urothelium Carcinogenesis.
Author: Reis, Leonardo O
Fávaro, Wagner J
Ferreira, Ubirajara
Billis, Athanase
Fazuoli, Mariana G
Cagnon, Valéria H A
Abstract: No optimal, well designed and reproducible animal model for upper urothelial carcinogenesis exists. This study characterized the histopathological features on top of immunolocalization of alpha-dystroglycans (alpha-DG) and matrix metalloproteinase (MMP-9) and cell turn-over in the upper urinary tract using a novel experimental model. Seventy-five female Fischer 344 rats were divided into three groups: the control group received a 0.30-ml dose of 0.9% physiological saline; the MNU group (chemical carcinogen N-methyl-N-nitrosourea) received 0.30 ml of MNU; and the MNU-citrate group received 0.30 ml of MNU plus sodium citrate, every one intravesically every other week for a total of 4 doses. After 15 weeks, bladder, ureters and renal pelvis were collected for morphological and molecular analysis. Associated management with MNU and sodium citrate was able to lead to 100% of both urinary bladder and upper urinary tract tumors, being the high-grade noninvasive papillary urothelial carcinoma the most frequent lesion. The upper urothelium showed reduced alpha-DG and increased MMP-9 and Ki-67 immunoreactivities in the MNU-citrate group in relation to the other groups. MNU group presented no upper urothelium tumor and 100% bladder tumor. This is a relevant evolution on experimental animal model for upper urinary tract carcinogenesis field. MMP-dependent disruption of the DG complex plays an important role in urothelial tumor carcinogenesis and showed the model applicability and significance. MNU-citrate model could contribute to a better understanding of human upper urothelial cancer development as well as to its local treatment strategies in a near future.
Subject: Alkylating Agents
Disease Models, Animal
Kidney Medulla
Rats, Inbred F344
Urinary Bladder
Urologic Neoplasms
Citation: World Journal Of Urology. v. 28, n. 4, p. 499-505, 2010-Aug.
Rights: fechado
Identifier DOI: 10.1007/s00345-010-0545-3
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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