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Type: Artigo de periódico
Title: Acute Exercise Reverses Aged-induced Impairments In Insulin Signaling In Rodent Skeletal Muscle.
Author: Pauli, José R
Ropelle, Eduardo R
Cintra, Dennys Esper
De Souza, Cláudio Teodoro
da Silva, Adelino S R
Moraes, Juliana C
Prada, Patricia O
de Almeida Leme, José A C
Luciano, Eliete
Velloso, Lício A
Carvalheira, José B C
Saad, Mario J A
Abstract: The insulin resistance associated with aging is improved by exercise, but the molecular mechanisms of this improvement are not fully understood. We investigated whether the improvement in insulin action, associated with acute exercise in old rats is dependent on the modulation of pIRS-1Ser307, JNK, IkBalpha and PTP-1B. Aging rats were subjected to swimming for two 1.5-h long bouts, separated by a 45min rest period. Sixteen hours after the exercise, the rats were killed and proteins from the insulin signaling pathway were analyzed by immunoblotting. Our results show that the reduction in glucose disappearance rate (Kitt), observed in aged rats, was restored at 16h after exercise. Aging led to an increase in Ser307 phosphorylation of IRS-1, and this was reversed by exercise in the skeletal muscle, in parallel with a reduction in pJNK and IkBalpha degradation. Moreover, aging induced an increase in the expression of PTP-1B and attenuated insulin signaling in the muscle of rats, a phenomenon that was reversed by exercise. Interestingly, the decrease in PTP-1B expression in the muscle of exercised old rats was accompanied by an increase in SIRT1 expression. These results provide new insights into the mechanisms by which exercise restores insulin sensitivity during aging.
Subject: Aging
I-kappa B Proteins
Insulin Receptor Substrate Proteins
Insulin Resistance
Map Kinase Kinase 4
Muscle, Skeletal
Physical Conditioning, Animal
Protein Tyrosine Phosphatase, Non-receptor Type 1
Rats, Wistar
Signal Transduction
Sirtuin 1
Citation: Mechanisms Of Ageing And Development. v. 131, n. 5, p. 323-9, 2010-May.
Rights: fechado
Identifier DOI: 10.1016/j.mad.2010.03.004
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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