Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198758
Type: Artigo de periódico
Title: Effect Of The Synthetic Coumarin, Ethyl 2-oxo-2h-chromene-3-carboxylate, On Activity Of Crotalus Durissus Ruruima Spla2 As Well As On Edema And Platelet Aggregation Induced By This Factor.
Author: Fonseca, F V
Baldissera, L
Camargo, E A
Antunes, E
Diz-Filho, E B S
Corrêa, A G
Beriam, L O S
Toyama, D O
Cotrim, C A
Alvin, Joel
Toyama, M H
Abstract: We show that ethyl 2-oxo-2H-chromene-3-carboxylate (EOCC), a synthetic coumarin, irreversibly inhibits phospholipase A(2) (sPLA2) from Crotalus durissus ruruima venom (sPLA2r) with an IC(50) of 3.1 +/- 0.06 nmol. EOCC strongly decreased the V(max) and K(m), and it virtually abolished the enzyme activity of sPLA2r as well as sPLA2s from other sources. The edema induced by sPLA2r + EOCC was less than that induced by sPLA2r treated with p-bromophenacyl bromide, which was more efficient at neutralizing the platelet aggregation activity of native sPLA2r. Native sPLA2r induced platelet aggregation of 91.54 +/- 9.3%, and sPLA2r + EOCC induced a platelet aggregation of 18.56 +/- 6.5%. EOCC treatment also decreased the myotoxic effect of sPLA2r. Mass spectrometry showed that EOCC formed a stable complex with sPLA2r, which increased the mass of native sPLA2r from 14,299.34 Da to 14,736.22 Da. Moreover, the formation of this complex appeared to be involved in the loss of sPLA2r activity. Our results strongly suggest that EOCC can be used as a pharmacological agent against the sPLA2 in Crotalus durissus sp. venom as well as other sPLA2s.
Subject: Animals
Antivenins
Coumarins
Crotalid Venoms
Crotalus
Edema
Enzyme Inhibitors
Male
Phospholipase A2 Inhibitors
Phospholipases A2
Platelet Aggregation
Rats
Rats, Wistar
Citation: Toxicon : Official Journal Of The International Society On Toxinology. v. 55, n. 8, p. 1527-30, 2010-Jul.
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2010.03.004
Address: http://www.ncbi.nlm.nih.gov/pubmed/20223257
Date Issue: 2010
Appears in Collections:Unicamp - Artigos e Outros Documentos

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