Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198490
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDifferent Mechanisms Underlie The Effects Of Acute And Long-term Inhibition Of Nitric Oxide Synthases In Antigen-induced Pulmonary Eosinophil Recruitment In Balb/c Mice.pt_BR
dc.contributor.authorLintomen, Leticiapt_BR
dc.contributor.authorSouza-Filho, Luis Gustavopt_BR
dc.contributor.authorFerreira, Tatianept_BR
dc.contributor.authorCamargo, Enilton Apt_BR
dc.contributor.authorTeixeira, Simone Apt_BR
dc.contributor.authorMuscará, Marcelo Npt_BR
dc.contributor.authorLandgraf, Richardt Gpt_BR
dc.contributor.authorJancar, Soniapt_BR
dc.contributor.authorMendes, Gustavo Dpt_BR
dc.contributor.authorDe Nucci, Gilbertopt_BR
dc.contributor.authorAntunes, Edsonpt_BR
unicamp.authorLeticia Lintomen, Department of Pharmacology, State University of Campinas (UNICAMP), Faculty of Medical Sciences, PO Box 6111, 13084-971 Campinas, São Paulo, Brazil.pt_BR
unicamp.author.externalLuis Gustavo Souza-Filho,pt
unicamp.author.externalTatiane Ferreira,pt
unicamp.author.externalEnilton A Camargo,pt
unicamp.author.externalSimone A Teixeira,pt
unicamp.author.externalMarcelo N Muscará,pt
unicamp.author.externalRichardt G Landgraf,pt
unicamp.author.externalSonia Jancar,pt
unicamp.author.externalGustavo D Mendes,pt
unicamp.author.externalGilberto De Nucci,pt
unicamp.author.externalEdson Antunes,pt
dc.subjectAdministration, Oralpt_BR
dc.subjectAnimalspt_BR
dc.subjectBiological Availabilitypt_BR
dc.subjectBrainpt_BR
dc.subjectBronchoalveolar Lavage Fluidpt_BR
dc.subjectDose-response Relationship, Drugpt_BR
dc.subjectDrug Administration Schedulept_BR
dc.subjectEnzyme Inhibitorspt_BR
dc.subjectEosinophilspt_BR
dc.subjectLungpt_BR
dc.subjectMalept_BR
dc.subjectMicept_BR
dc.subjectMice, Inbred Balb Cpt_BR
dc.subjectNg-nitroarginine Methyl Esterpt_BR
dc.subjectNitric Oxide Synthasept_BR
dc.subjectNitric Oxide Synthase Type Iipt_BR
dc.subjectOvalbuminpt_BR
dc.description.abstractNitric oxide synthase (NOS) inhibitors are largely used to evaluate the NO contribution to pulmonary allergy, but contrasting data have been reported. In this study, pharmacological, biochemical and pharmacokinetic assays were performed to compare the effects of acute and long-term treatment of BALB/C mice with the non-selective NOS inhibitor L-NAME in ovalbumin (OVA)-challenged mice. Acute L-NAME treatment (50 mg/kg, gavage) significantly reduced the eosinophil number in bronchoalveolar lavage fluid (BALF). The inducible NOS (iNOS) inhibitor aminoguanidine (20 mg/kg/day in the drinking water) also significantly reduced the eosinophil number in BALF. In contrast, 3-week L-NAME treatment (50 and 150 mg/kg/day in the drinking water) significantly increased the pulmonary eosinophil influx. The constitutive NOS (cNOS) activity in brain and lungs was reduced by both acute and 3-week L-NAME treatments. The pulmonary iNOS activity was reduced by acute L-NAME (or aminoguanidine), but unaffected by 3-week L-NAME treatment. Acute L-NAME (or aminoguanidine) treatment was more efficient to reduce the NOx- levels compared with 3-week L-NAME treatment. The pharmacokinetic study revealed that L-NAME is not bioavailable when given orally. After acute L-NAME intake, serum concentrations of the metabolite Nomega-nitro-L-arginine decreased from 30 min to 24 h. In the 3-week L-NAME treatment, the Nomega-nitro-L-arginine concentration was close to the detection limit. In conclusion, 3-week treatment with l-NAME yields low serum Nomega-nitro-L-arginine concentrations, causing preferential inhibition of cNOS activity. Therefore, eosinophil influx potentiation by 3-week L-NAME treatment may reflect removal of protective cNOS-derived NO, with no interference on the ongoing inflammation due to iNOS-derived NO.en
dc.relation.ispartofPulmonary Pharmacology & Therapeuticspt_BR
dc.relation.ispartofabbreviationPulm Pharmacol Therpt_BR
dc.date.issued2009-Febpt_BR
dc.identifier.citationPulmonary Pharmacology & Therapeutics. v. 22, n. 1, p. 1-8, 2009-Feb.pt_BR
dc.language.isoengpt_BR
dc.description.volume22pt_BR
dc.description.firstpage1-8pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1094-5539pt_BR
dc.identifier.doi10.1016/j.pupt.2008.10.003pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/19010435pt_BR
dc.date.available2015-11-27T13:15:35Z-
dc.date.accessioned2015-11-27T13:15:35Z-
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dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198490-
dc.identifier.idPubmed19010435pt_BR
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