Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/198369
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleAscorbate-induced Osteoblast Differentiation Recruits Distinct Mmp-inhibitors: Reck And Timp-2.pt_BR
dc.contributor.authorZambuzzi, Willian Fpt_BR
dc.contributor.authorYano, Claudia Lpt_BR
dc.contributor.authorCavagis, Alexandre D Mpt_BR
dc.contributor.authorPeppelenbosch, Maikel Ppt_BR
dc.contributor.authorGranjeiro, José Mauropt_BR
dc.contributor.authorFerreira, Carmen Vpt_BR
unicamp.authorWillian F Zambuzzi, Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Cidade Universitária, 13083-970, Campinas, Sao Paulo, Brazil. wzamba@unicamp.brpt_BR
unicamp.author.externalClaudia L Yano,pt
unicamp.author.externalAlexandre D M Cavagis,pt
unicamp.author.externalMaikel P Peppelenbosch,pt
unicamp.author.externalJosé Mauro Granjeiro,pt
unicamp.author.externalCarmen V Ferreira,pt
dc.subject3t3 Cellspt_BR
dc.subjectAnimalspt_BR
dc.subjectAscorbic Acidpt_BR
dc.subjectCell Cyclept_BR
dc.subjectCell Differentiationpt_BR
dc.subjectCells, Culturedpt_BR
dc.subjectExtracellular Matrixpt_BR
dc.subjectGpi-linked Proteinspt_BR
dc.subjectMembrane Glycoproteinspt_BR
dc.subjectMicept_BR
dc.subjectOsteoblastspt_BR
dc.subjectProtein Kinase Cpt_BR
dc.subjectProtein Tyrosine Phosphatase, Non-receptor Type 11pt_BR
dc.subjectSignal Transductionpt_BR
dc.subjectTissue Inhibitor Of Metalloproteinase-2pt_BR
dc.subjectRac1 Gtp-binding Proteinpt_BR
dc.subjectRho Gtp-binding Proteinspt_BR
dc.description.abstractThe bone formation executed by osteoblasts represents an interesting research field both for basic and applied investigations. The goal of this work was to evaluate the molecular mechanisms involved during osteoblast differentiation in vitro. Accordingly, we demonstrated that, during the osteoblastic differentiation, TIMP-2 and RECK presented differential expressions, where RECK expression was downregulated from the 14th day in contrast with an increase in TIMP-2. Concomitantly, our results showed a temporal regulation of two major signaling cascades during osteoblast differentiation: proliferation cascades in which RECK, PI3 K, and GSK-3beta play a pivotal role and latter, differentiation cascades with participation of Ras, Rho, Rac-1, PKC alpha/beta, and TIMP-2. Furthermore, we observed that phosphorylation level of paxillin was downregulated while FAK(125) remained unchangeable, but active during extracellular matrix (ECM) remodeling. Concluding, our results provide evidences that RECK and TIMP-2 are involved in the control of ECM remodeling in distinct phases of osteoblast differentiation by modulating MMP activities and a multitude of signaling proteins governs these events.en
dc.relation.ispartofMolecular And Cellular Biochemistrypt_BR
dc.relation.ispartofabbreviationMol. Cell. Biochem.pt_BR
dc.date.issued2009-Febpt_BR
dc.identifier.citationMolecular And Cellular Biochemistry. v. 322, n. 1-2, p. 143-50, 2009-Feb.pt_BR
dc.language.isoengpt_BR
dc.description.volume322pt_BR
dc.description.firstpage143-50pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1573-4919pt_BR
dc.identifier.doi10.1007/s11010-008-9951-xpt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/18989628pt_BR
dc.date.available2015-11-27T13:15:20Z-
dc.date.accessioned2015-11-27T13:15:20Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:15:20Z (GMT). No. of bitstreams: 1 pmed_18989628.pdf: 395542 bytes, checksum: 08f057bbc0e09d041b39e6b7ef01cfe6 (MD5) Previous issue date: 2009en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/198369-
dc.identifier.idPubmed18989628pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_18989628.pdf386.27 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.