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dc.typeArtigo de periódicopt_BR
dc.titleAscorbate-induced Osteoblast Differentiation Recruits Distinct Mmp-inhibitors: Reck And Timp-2.pt_BR
dc.contributor.authorZambuzzi, Willian Fpt_BR
dc.contributor.authorYano, Claudia Lpt_BR
dc.contributor.authorCavagis, Alexandre D Mpt_BR
dc.contributor.authorPeppelenbosch, Maikel Ppt_BR
dc.contributor.authorGranjeiro, José Mauropt_BR
dc.contributor.authorFerreira, Carmen Vpt_BR
unicamp.authorWillian F Zambuzzi, Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Cidade Universitária, 13083-970, Campinas, Sao Paulo, Brazil. wzamba@unicamp.brpt_BR L Yano,pt D M Cavagis,pt P Peppelenbosch,pté Mauro Granjeiro,pt V Ferreira,pt
dc.subject3t3 Cellspt_BR
dc.subjectAscorbic Acidpt_BR
dc.subjectCell Cyclept_BR
dc.subjectCell Differentiationpt_BR
dc.subjectCells, Culturedpt_BR
dc.subjectExtracellular Matrixpt_BR
dc.subjectGpi-linked Proteinspt_BR
dc.subjectMembrane Glycoproteinspt_BR
dc.subjectProtein Kinase Cpt_BR
dc.subjectProtein Tyrosine Phosphatase, Non-receptor Type 11pt_BR
dc.subjectSignal Transductionpt_BR
dc.subjectTissue Inhibitor Of Metalloproteinase-2pt_BR
dc.subjectRac1 Gtp-binding Proteinpt_BR
dc.subjectRho Gtp-binding Proteinspt_BR
dc.description.abstractThe bone formation executed by osteoblasts represents an interesting research field both for basic and applied investigations. The goal of this work was to evaluate the molecular mechanisms involved during osteoblast differentiation in vitro. Accordingly, we demonstrated that, during the osteoblastic differentiation, TIMP-2 and RECK presented differential expressions, where RECK expression was downregulated from the 14th day in contrast with an increase in TIMP-2. Concomitantly, our results showed a temporal regulation of two major signaling cascades during osteoblast differentiation: proliferation cascades in which RECK, PI3 K, and GSK-3beta play a pivotal role and latter, differentiation cascades with participation of Ras, Rho, Rac-1, PKC alpha/beta, and TIMP-2. Furthermore, we observed that phosphorylation level of paxillin was downregulated while FAK(125) remained unchangeable, but active during extracellular matrix (ECM) remodeling. Concluding, our results provide evidences that RECK and TIMP-2 are involved in the control of ECM remodeling in distinct phases of osteoblast differentiation by modulating MMP activities and a multitude of signaling proteins governs these events.en
dc.relation.ispartofMolecular And Cellular Biochemistrypt_BR
dc.relation.ispartofabbreviationMol. Cell. Biochem.pt_BR
dc.identifier.citationMolecular And Cellular Biochemistry. v. 322, n. 1-2, p. 143-50, 2009-Feb.pt_BR
dc.description.provenanceMade available in DSpace on 2015-11-27T13:15:20Z (GMT). No. of bitstreams: 1 pmed_18989628.pdf: 395542 bytes, checksum: 08f057bbc0e09d041b39e6b7ef01cfe6 (MD5) Previous issue date: 2009en
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