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Type: Artigo de periódico
Title: The Untiring Search For The Most Complete Proteome Representation: Reviewing The Methods.
Author: Martins de Souza, Daniel
Oliveira, Bruno M
Castro-Dias, Erich
Winck, Flavia V
Horiuchi, Ricardo S O
Baldasso, Paulo A
Caetano, Hugo T
Pires, Natália Katauchi Dayane
Marangoni, Sergio
Novello, José C
Abstract: Proteomic research has proved valuable for understanding the molecular mechanisms of biological processes, as well as in the search for biomarkers for a variety of diseases which lack a molecular diagnostic. While several new approaches are being developed, two-dimensional (2-DE) gel electrophoresis is still one of the most commonly used techniques, despite its many limitations. However, for biomarker research, 2-DE gel electrophoresis alone does not fulfill the necessary pre-requisites. If such a technique is utilized exclusively, a great part of a given proteome remains unseen. Therefore, very precise and sensitive techniques are needed. Here, we present a brief review of known methodologies that try to overcome the limitations of conventional proteome analysis as well as their respective advantages and limitations.
Subject: Animals
Electrophoresis, Gel, Two-dimensional
Gene Expression Profiling
Nerve Tissue Proteins
Research Design
Sensitivity And Specificity
Citation: Briefings In Functional Genomics & Proteomics. v. 7, n. 4, p. 312-21, 2008-Jul.
Rights: fechado
Identifier DOI: 10.1093/bfgp/eln023
Date Issue: 2008
Appears in Collections:Unicamp - Artigos e Outros Documentos

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