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Type: Artigo de periódico
Title: Expression Of Hypoxia-inducible Factor 1alpha In Mononuclear Phagocytes Infected With Leishmania Amazonensis.
Author: Degrossoli, Adriana
Bosetto, Maira Cegatti
Lima, Camila Bárbara Cantalupo
Giorgio, Selma
Abstract: Increasing evidence indicates that hypoxia-inducible factor 1alpha (HIF-1alpha) can be upregulated in different cell types by nonhypoxic stimuli such as growth factors, cytokines, nitric oxide, lipopolysaccharides and a range of infectious microorganisms. In this study, the ability of the following mononuclear phagocytes to express HIF-1alpha is reported: mouse macrophages (mMPhi), human macrophages (hMPhi) and human dendritic cells (DC), parasitized in vitro with Leishmania amazonensis; as assessed by immunofluorescence microscopy. A logical explanation for HIF-1alpha expression might be that the mononuclear phagocytes became hypoxic after L. amazonensis infection. Using the hypoxia marker pimonidazole, observation revealed that L. amazonensis-infected cells were not hypoxic. In addition, experiments using a HIF-1alpha inhibitor, CdCl(2), to treat L. amazonensis-infected macrophage cultures showed reduced parasite survival. These studies indicated that HIF-1alpha could play a role in adaptative and immune responses of mononuclear phagocytes presenting infection by the parasite L. amazonensis.
Subject: Animals
Cadmium Chloride
Cell Hypoxia
Cells, Cultured
Dendritic Cells
Hypoxia-inducible Factor 1, Alpha Subunit
Leishmania Mexicana
Macrophages, Peritoneal
Mice, Inbred Balb C
Citation: Immunology Letters. v. 114, n. 2, p. 119-25, 2007-Dec.
Rights: fechado
Identifier DOI: 10.1016/j.imlet.2007.09.009
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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