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Type: Artigo de periódico
Title: 17-beta-estradiol Affects Nuclear Image Properties In Mcf-10f Human Breast Epithelial Cells With Tumorigenesis.
Author: Mello, Maria Luiza S
Russo, Patricia
Russo, Jose
Vidal, Benedicto C
Abstract: Treatment of the human breast epithelial cells MCF-10F with 17-beta-estradiol (E2) induces transformation and tumorigenesis. E2-transformed MCF-10F cells are known to exhibit progressive loss of ductulogenesis, and invasive and tumorigenic phenotypes. Although DNA amounts and chromatin supraorganization change in E2-transformed MCF cells, no comparative study has yet been undertaken in the resulting cells selected for aggressive invasiveness (C5) and tumor generation in a heterologous host. The aim of this study was thus to determine whether changes in Feulgen-DNA content and chromatin supraorganization are involved during E2-induced transformation and tumorigenesis of the MCF-10F cells. Image analysis was performed for nontransformed and E2-transformed MCF cells, highly invasive cells (C5), and for cell lines (C5-A6-T6 and C5-A8-T8) derived from tumors generated by injection of C5 cells in SCID mice. A decrease in Feulgen-DNA amounts and nuclear sizes induced by E2 treatment was accented with selection of the highly invasive tumorigenesis potential. However, in the tumor-derived cells a high variability in cellular phenotypes resulted inclusively in near-polyploidy. Significant changes in textural parameters, including nuclear entropy, indicated chromatin structural remodeling with advancing tumorigenesis. An increased variability in the degree of chromatin packing states in the E2-transformed MCF cells is followed by reduction in chromatin condensation and in contrast between condensed and noncondensed chromatin in the highly invasive C5 cells and tumor-derived cell lines. Studies on epigenetic mechanisms involving DNA methylation and/or the histone code would contribute to a better interpretation of the chromatin supraorganization changes reported herein.
Subject: Breast
Breast Neoplasms
Cell Line, Tumor
Cell Nucleus
Epithelial Cells
Neoplasm Invasiveness
Citation: Oncology Reports. v. 18, n. 6, p. 1475-81, 2007-Dec.
Rights: fechado
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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