Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197329
Full metadata record
DC FieldValueLanguage
dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleExtra-pancreatic Insulin Production In Rat Lachrymal Gland After Streptozotocin-induced Islet Beta-cells Destruction.pt_BR
dc.contributor.authorCunha, Daniel Andradept_BR
dc.contributor.authorde Alves, Mônica Cpt_BR
dc.contributor.authorStoppiglia, Luiz Fabriziopt_BR
dc.contributor.authorJorge, Angélica Gobbipt_BR
dc.contributor.authorMódulo, Carolina Mariapt_BR
dc.contributor.authorCarneiro, Everardo Mpt_BR
dc.contributor.authorBoschero, Antonio Cpt_BR
dc.contributor.authorSaad, Mário J Apt_BR
dc.contributor.authorVelloso, Lício Apt_BR
dc.contributor.authorRocha, Eduardo Mpt_BR
unicamp.authorDaniel Andrade Cunha, Institute of Biology, State University of Campinas (UNICAMP), Campinas, SP, Brazil.pt_BR
unicamp.author.externalMônica C de Alves,pt
unicamp.author.externalLuiz Fabrizio Stoppiglia,pt
unicamp.author.externalAngélica Gobbi Jorge,pt
unicamp.author.externalCarolina Maria Módulo,pt
unicamp.author.externalEverardo M Carneiro,pt
unicamp.author.externalAntonio C Boschero,pt
unicamp.author.externalMário J A Saad,pt
unicamp.author.externalLício A Velloso,pt
unicamp.author.externalEduardo M Rocha,pt
dc.subjectAnimalspt_BR
dc.subjectCarbacholpt_BR
dc.subjectDiabetes Mellitus, Experimentalpt_BR
dc.subjectDose-response Relationship, Drugpt_BR
dc.subjectGlucosept_BR
dc.subjectImmunohistochemistrypt_BR
dc.subjectInsulinpt_BR
dc.subjectIslets Of Langerhanspt_BR
dc.subjectLacrimal Apparatuspt_BR
dc.subjectMalept_BR
dc.subjectPancreaspt_BR
dc.subjectPeroxidasept_BR
dc.subjectRatspt_BR
dc.subjectRats, Wistarpt_BR
dc.subjectStreptozocinpt_BR
dc.subjectSynaptophysinpt_BR
dc.description.abstractPrevious work has revealed that insulin is secreted in the tear film; its mRNA is expressed in the lachrymal gland (LG) and its receptor in tissues of the ocular surface. To test the hypothesis of insulin production in the LG, we compared normal and diabetic rats for: (1) the presence of insulin and C-peptide, (2) glucose- and carbachol-induced insulin secretion ex-vivo, and (3) biochemical and histological characteristics of diabetic LG that would support this possibility. Four weeks after streptozotocin injection, blood and tears were collected from streptozotocin-diabetic male Wistar rats. Insulin levels in the tear film rose after glucose stimulation in diabetic rats, but remained unchanged in the blood. Ex vivo static secretion assays demonstrated that higher glucose and 200 microM carbachol significantly increased mean insulin levels from LG samples of both groups. Insulin and C-peptide were expressed in LG of diabetic rats as determined by RIA. Comparable synaptophysin immune staining and peroxidase activity in the LG of both groups suggest that the structure and function of these tissues were maintained. These findings provide evidence of insulin production by LG. Higher expression of reactive oxygen species scavengers may prevent oxidative damage to LG compared to pancreatic beta-cells.en
dc.relation.ispartofBiochimica Et Biophysica Actapt_BR
dc.relation.ispartofabbreviationBiochim. Biophys. Actapt_BR
dc.date.issued2007-Augpt_BR
dc.identifier.citationBiochimica Et Biophysica Acta. v. 1770, n. 8, p. 1128-35, 2007-Aug.pt_BR
dc.language.isoengpt_BR
dc.description.volume1770pt_BR
dc.description.firstpage1128-35pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0006-3002pt_BR
dc.identifier.doi10.1016/j.bbagen.2007.05.002pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/17561349pt_BR
dc.date.available2015-11-27T13:10:11Z-
dc.date.accessioned2015-11-27T13:10:11Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:10:11Z (GMT). No. of bitstreams: 1 pmed_17561349.pdf: 1174468 bytes, checksum: aec777c554acbe8936f8bcc49067172d (MD5) Previous issue date: 2007en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197329-
dc.identifier.idPubmed17561349pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_17561349.pdf1.15 MBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.