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Type: Artigo de periódico
Title: Neuromuscular And Phospholipase Activities Of Venoms From Three Subspecies Of Bothrops Neuwiedi (b. N. Goyazensis, B. N. Paranaensis And B. N. Diporus).
Author: Abreu, Valdemir A
Dal Belo, Cháriston A
Hernandes-Oliveira, Saraguaci S
Borja-Oliveira, Caroline R
Hyslop, Stephen
Furtado, Maria de Fátima D
Rodrigues-Simioni, Léa
Abstract: The Bothrops neuwiedi (Neuwied's lancehead) species complex consists of a variety of subspecies with a wide distribution in South America. In this work, we compared the neuromuscular blockade caused by venoms from three subspecies (B. n. goyazensis, B. n. paranaensis and B. n. diporus) of this complex using chick biventer cervicis (BC) and mouse phrenic nerve-diaphragm (PND) preparations and investigated their phospholipase A2 (PLA2) activities and electrophoretic profiles. The order of potency of PLA2 activity was B. n. diporus>B. n. paranaensis>B. n. goyazensis. In BC preparations, B. n. goyazensis venom (50 microg/mL) was significantly (p<0.05) more active than B. n. paranaensis and B. n. diporus venoms, which did not produce a significant blockade at this time interval; after 120 min, B. n. goyazensis, B. n. paranaensis and B. n. diporus venoms (100 microg/mL) produced blockades of 57.4+/-5%, 30+/-3% and 17.4+/-7% (n=3-6 each), respectively. The three venoms inhibited contractures in response to ACh, indicating interference with postsynaptic neurotransmission. Only B. n. goyazensis and B. n. paranaensis venoms caused a long-lasting, concentration-dependent muscle contracture prior to blockade. In PND preparations, all of the venoms blocked the twitch-tension responses within 45-100 min, indicating that these preparations were more sensitive than avian preparations. There was a correlation between PLA2 activity and the time for 50% blockade in PND but not in BC preparations. SDS-PAGE showed quantitative rather than qualitative differences among the venoms. These results indicate that the venoms of the three subspecies had similar profiles of neuromuscular activity, although the relationship with PLA2 activity varied with the preparation used.
Subject: Animals
Electrophoresis, Polyacrylamide Gel
Phospholipases A
Phospholipases A2
Phrenic Nerve
Snake Venoms
Species Specificity
Citation: Comparative Biochemistry And Physiology. Part A, Molecular & Integrative Physiology. v. 148, n. 1, p. 142-9, 2007-Sep.
Rights: fechado
Identifier DOI: 10.1016/j.cbpa.2007.03.030
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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