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Type: Artigo de periódico
Title: Leishmania Amazonensis: Multiple Receptor-ligand Interactions Are Involved In Amastigote Infection Of Human Dendritic Cells.
Author: Bosetto, Maira Cegatti
Giorgio, Selma
Abstract: In their mammalian hosts, Leishmania are obligate intracellular parasites that reside in macrophages and dendritic cells (DCs). In the present study, we have investigated in vitro the mechanisms of entry into human DCs of Leishmania amazonensis amastigotes isolated from lesions in nude mice (Am nude). The DC infection rate with Am nude was approximately 36%, while opsonization of Am nude with normal human serum and infected human serum increased the DC infection rates to 60% and 62%, respectively. Heat inactivation and depletion of antibodies in sera brought the DC infection rate down to 40%. The DC infection rate was inhibited after pre-treatment of Am nude with heparin. We were unable to implicate mannose-fucose receptors in the uptake of Am nude by DCs. Our data suggest that the ability of L. amazonensis amastigotes to infect human DCs involves the participation of at least three multiple receptor-ligand interactions, antibodies/FcR, complement components/CR and proteoglycans/heparin-binding protein.
Subject: Animals
Antibodies, Protozoan
Cell Adhesion Molecules
Cells, Cultured
Complement System Proteins
Dendritic Cells
Heparitin Sulfate
Immune Sera
Lectins, C-type
Leishmania Mexicana
Mannose-binding Lectins
Mice, Inbred C3h
Mice, Nude
Opsonin Proteins
Receptors, Cell Surface
Receptors, Complement
Receptors, Fc
Citation: Experimental Parasitology. v. 116, n. 3, p. 306-10, 2007-Jul.
Rights: fechado
Identifier DOI: 10.1016/j.exppara.2007.01.003
Date Issue: 2007
Appears in Collections:Unicamp - Artigos e Outros Documentos

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