Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/197161
Type: Artigo de periódico
Title: The Plant Energy-dissipating Mitochondrial Systems: Depicting The Genomic Structure And The Expression Profiles Of The Gene Families Of Uncoupling Protein And Alternative Oxidase In Monocots And Dicots.
Author: Borecky, Jirí
Nogueira, Fábio T S
de Oliveira, Kívia A P
Maia, Ivan G
Vercesi, Aníbal E
Arruda, Paulo
Abstract: The simultaneous existence of alternative oxidases and uncoupling proteins in plants has raised the question as to why plants need two energy-dissipating systems with apparently similar physiological functions. A probably complete plant uncoupling protein gene family is described and the expression profiles of this family compared with the multigene family of alternative oxidases in Arabidopsis thaliana and sugarcane (Saccharum sp.) employed as dicot and monocot models, respectively. In total, six uncoupling protein genes, AtPUMP1-6, were recognized within the Arabidopsis genome and five (SsPUMP1-5) in a sugarcane EST database. The recombinant AtPUMP5 protein displayed similar biochemical properties as AtPUMP1. Sugarcane possessed four Arabidopsis AOx1-type orthologues (SsAOx1a-1d); no sugarcane orthologue corresponding to Arabidopsis AOx2-type genes was identified. Phylogenetic and expression analyses suggested that AtAOx1d does not belong to the AOx1-type family but forms a new (AOx3-type) family. Tissue-enriched expression profiling revealed that uncoupling protein genes were expressed more ubiquitously than the alternative oxidase genes. Distinct expression patterns among gene family members were observed between monocots and dicots and during chilling stress. These findings suggest that the members of each energy-dissipating system are subject to different cell or tissue/organ transcriptional regulation. As a result, plants may respond more flexibly to adverse biotic and abiotic conditions, in which oxidative stress is involved.
Subject: Amino Acid Sequence
Arabidopsis
Carrier Proteins
Cold Temperature
Computational Biology
Gene Expression Profiling
Genome, Plant
Ion Channels
Membrane Proteins
Mitochondria
Mitochondrial Proteins
Molecular Sequence Data
Multigene Family
Oxidoreductases
Phylogeny
Plant Proteins
Rna, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Saccharum
Sequence Alignment
Sequence Analysis, Protein
Citation: Journal Of Experimental Botany. v. 57, n. 4, p. 849-64, 2006.
Rights: fechado
Identifier DOI: 10.1093/jxb/erj070
Address: http://www.ncbi.nlm.nih.gov/pubmed/16473895
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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