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Type: Artigo de periódico
Title: The Putative Telomerase Reverse Transcriptase Component Of Leishmania Amazonensis: Gene Cloning And Characterization.
Author: Giardini, Miriam A
Lira, Cristina B B
Conte, Fábio F
Camillo, Luciana R
de Siqueira Neto, Jair L
Ramos, Carlos H I
Cano, Maria Isabel N
Abstract: The Leishmania amazonensis telomerase gene was cloned by a polymerase chain reaction-based strategy using primers designed from a Leishmania major sequence that shared similarities with conserved telomerase motifs. The genes from three other species were cloned for comparative purposes. A ClustalW multiple-sequence alignment demonstrated that the Leishmania telomerases show greater homology with each other than with the proteins of other kinetoplastids and eukaryotes. Characterization experiments indicated that the putative Leishmania telomerase gene was probably in single copy and located in the largest chromosomes. A single messenger ribonucleic acid transcript was found in promastigotes. Phylogenetic analysis suggested that Leishmania telomerase might represent a liaison between the oldest and the newest branches of telomerases.
Subject: Amino Acid Sequence
Chromosome Mapping
Cloning, Molecular
Dna Primers
Dna, Protozoan
Evolution, Molecular
Leishmania Major
Leishmania Mexicana
Molecular Sequence Data
Polymerase Chain Reaction
Rna, Messenger
Sequence Alignment
Sequence Analysis, Dna
Sequence Homology, Amino Acid
Citation: Parasitology Research. v. 98, n. 5, p. 447-54, 2006-Apr.
Rights: fechado
Identifier DOI: 10.1007/s00436-005-0036-4
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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