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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.title[the Effect Of Bacterial Lipopolysaccharide On The Gastric Emptying Of Rats: A Pretreatment Evaluation Using Nw-nitro-l-arginine Methyl Ester (l-name)].pt_BR
dc.contributor.authorCollares, Edgard Ferropt_BR
dc.contributor.authorVinagre, Adriana Mendespt_BR
unicamp.authorEdgard Ferro Collares, Centro de Investigação em Pediatria, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, SP. efcollares@hotmail.compt_BR
unicamp.author.externalAdriana Mendes Vinagre,pt
dc.subjectAnalysis Of Variancept_BR
dc.subjectAnimalspt_BR
dc.subjectDisease Models, Animalpt_BR
dc.subjectEnzyme Inhibitorspt_BR
dc.subjectGastric Emptyingpt_BR
dc.subjectLipopolysaccharidespt_BR
dc.subjectMalept_BR
dc.subjectNg-nitroarginine Methyl Esterpt_BR
dc.subjectNitric Oxide Synthasept_BR
dc.subjectRatspt_BR
dc.subjectRats, Wistarpt_BR
dc.description.abstractThere is evidence that nitric oxide plays a role in the decrease in gastric emptying induced by bacterial lipopolysaccharide. To evaluate the effect of pretreatment with Nw-nitro-L-arginine methyl to ester, one competitive inhibitor of the nitric oxide synthases, on the gastric emptying delay induced by lipopolysaccharide. Male Wistar rats, SPF, were used after 24 h fast and 1 h-water withdrawn. The pretreatment was done intravenously with vehicle (saline) or N(w)-nitro-L-arginine methyl to ester in the doses of 0.5, 1, 2.5 e 5 mg/kg. After 10 min, the animals were treated iv with lipopolysaccharide (50 microg/kg) or received vehicle (saline). The gastric emptying was evaluated 1 h after the lipopolysaccharide administration. A saline solution containing phenol red was used as the test meal. The gastric emptying was indirectly assessed by the determination of percent gastric retention of the test meal 10 min after orogastric administration. The animals pretreated with vehicle and treatment with lipopolysaccharide have significant rise of the gastric retention (average = 57%) in comparison with the controls receiving only vehicle (38.1%). The pretreatment with the different doses of N(w)-nitro-L-arginine methyl to ester did not modify per se the gastric retention in comparison with the animals pretreated with vehicle. Pretreatment with N(w)-nitro-L-arginine methyl to ester with the dose of 1 mg/kg determined a discrete but significant reduction in the gastric retention (52%) of animals treated with lipopolysaccharide in comparison with vehicle-pretreated rats. Paradoxically, animals pretreated with 2.5 or 5 mg of N(w)-nitro-L-arginine methyl to ester/kg followed by treatment with lipopolysaccharide displayed a significantly higher gastric retention (74.7% and 80.5%, respectively) as compared to their controls, pretreated with the same doses of the inhibitor and treated with vehicle (40.5% and 38.7%, respectively) and to those pretreated with vehicle and treated with the same toxin. The pretreatment with N(w)-nitro-L-arginine methyl to ester at low dose (1 mg/kg) resulted in a discrete inhibition of the gastric emptying delay induced by lipopolysaccharide. Nevertheless, N(w)-nitro-L-arginine methyl to ester at higher doses (2.5 and 5 mg/kg) induced an enhancement of the lipopolysaccharide effect on gastric emptying, despite not interfering with the gastric emptying per se.en
dc.relation.ispartofArquivos De Gastroenterologiapt_BR
dc.relation.ispartofabbreviationArq Gastroenterolpt_BR
dc.identifier.citationArquivos De Gastroenterologia. v. 43, n. 3, p. 229-32pt_BR
dc.language.isoporpt_BR
dc.description.volume43pt_BR
dc.description.firstpage229-32pt_BR
dc.rightsabertopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0004-2803pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/17160240pt_BR
dc.date.available2015-11-27T13:06:05Z-
dc.date.accessioned2015-11-27T13:06:05Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:06:05Z (GMT). No. of bitstreams: 1 pmed_17160240.pdf: 97064 bytes, checksum: e4c1b851a65d4ae5741ebe652b53db16 (MD5) Previous issue date: nullen
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/197038-
dc.identifier.idPubmed17160240pt_BR
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