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Type: Artigo de periódico
Title: Fetal Rhd Genotyping From Maternal Plasma In A Population With A Highly Diverse Ethnic Background.
Author: Machado, Isabela Nelly
Castilho, Lilian
Pellegrino, Jordão
Barini, Ricardo
Abstract: To establish the performance of conventional PCR as a noninvasive method for fetal genotyping, by free fetal DNA analysis of distinct RHD regions from maternal plasma, in a population of a diverse ethnic origin. We conducted a validity of the diagnostic test by analyzing 81 plasma samples from RhD-negative Brazilian pregnant women, from 4 to 41 gestational weeks. We tested for exon 10 and intron 4 gene regions by allele specific-PCR. Fetal RHD genotyping by PCR on maternal plasma was compared to serologic RhD typing in the neonatal period. Samples were obtained as follows: 15 in the 1st, 37 in the 2nd and 29 in the 3rd trimester. General accuracy was 97.3%, sensitivity of 98.3% and specificity of 93.8%. Conventional PCR is an accurate method for fetal RHD genotyping on maternal plasma, even in a population of mixed ethnic origin.
Subject: Brazil
Electrophoresis, Agar Gel
Ethnic Groups
Fetal Blood
Genetic Variation
Polymerase Chain Reaction
Pregnancy Trimesters
Prenatal Diagnosis
Rh-hr Blood-group System
Sensitivity And Specificity
Citation: Revista Da Associação Médica Brasileira (1992). v. 52, n. 4, p. 232-5
Rights: fechado
Appears in Collections:Unicamp - Artigos e Outros Documentos

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