Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196907
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleMitochondrial Dna Damage Associated With Lipid Peroxidation Of The Mitochondrial Membrane Induced By Fe2+-citrate.pt_BR
dc.contributor.authorAlmeida, Andréa Mpt_BR
dc.contributor.authorBertoncini, Clélia R Apt_BR
dc.contributor.authorBorecký, Jiript_BR
dc.contributor.authorSouza-Pinto, Nadja Cpt_BR
dc.contributor.authorVercesi, Aníbal Ept_BR
unicamp.authorAndréa M Almeida, Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brasil.pt_BR
unicamp.author.externalClélia R A Bertoncini,pt
unicamp.author.externalJiri Borecký,pt
unicamp.author.externalNadja C Souza-Pinto,pt
unicamp.author.externalAníbal E Vercesi,pt
dc.subjectAnimalspt_BR
dc.subjectDna Damagept_BR
dc.subjectDna, Mitochondrialpt_BR
dc.subjectFerrous Compoundspt_BR
dc.subjectLipid Peroxidationpt_BR
dc.subjectMalept_BR
dc.subjectMitochondria, Liverpt_BR
dc.subjectMitochondrial Membranespt_BR
dc.subjectMitochondrial Swellingpt_BR
dc.subjectRatspt_BR
dc.subjectRats, Wistarpt_BR
dc.description.abstractIron imbalance/accumulation has been implicated in oxidative injury associated with many degenerative diseases such as hereditary hemochromatosis, beta-thalassemia, and Friedreich's ataxia. Mitochondria are particularly sensitive to iron-induced oxidative stress - high loads of iron cause extensive lipid peroxidation and membrane permeabilization in isolated mitochondria. Here we detected and characterized mitochondrial DNA damage in isolated rat liver mitochondria exposed to a Fe2+-citrate complex, a small molecular weight complex. Intense DNA fragmentation was induced after the incubation of mitochondria with the iron complex. The detection of 3' phosphoglycolate ends at the mtDNA strand breaks by a 32P-postlabeling assay, suggested the involvement of hydroxyl radical in the DNA fragmentation induced by Fe2+-citrate. Increased levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine also suggested that Fe2+-citrate-induced oxidative stress causes mitochondrial DNA damage. In conclusion, our results show that iron-mediated lipid peroxidation was associated with intense mtDNA damage derived from the direct attack of reactive oxygen species.en
dc.relation.ispartofAnais Da Academia Brasileira De Ciênciaspt_BR
dc.relation.ispartofabbreviationAn. Acad. Bras. Cienc.pt_BR
dc.date.issued2006-Seppt_BR
dc.identifier.citationAnais Da Academia Brasileira De Ciências. v. 78, n. 3, p. 505-14, 2006-Sep.pt_BR
dc.language.isoengpt_BR
dc.description.volume78pt_BR
dc.description.firstpage505-14pt_BR
dc.rightsabertopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0001-3765pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/16936939pt_BR
dc.date.available2015-11-27T13:05:45Z-
dc.date.accessioned2015-11-27T13:05:45Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:05:45Z (GMT). No. of bitstreams: 1 pmed_16936939.pdf: 207230 bytes, checksum: 1da3b9c5284053f1f4e123c97f2754c6 (MD5) Previous issue date: 2006en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/196907-
dc.identifier.idPubmed16936939pt_BR
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