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Type: Artigo de periódico
Title: Mitochondrial Dna Damage Associated With Lipid Peroxidation Of The Mitochondrial Membrane Induced By Fe2+-citrate.
Author: Almeida, Andréa M
Bertoncini, Clélia R A
Borecký, Jiri
Souza-Pinto, Nadja C
Vercesi, Aníbal E
Abstract: Iron imbalance/accumulation has been implicated in oxidative injury associated with many degenerative diseases such as hereditary hemochromatosis, beta-thalassemia, and Friedreich's ataxia. Mitochondria are particularly sensitive to iron-induced oxidative stress - high loads of iron cause extensive lipid peroxidation and membrane permeabilization in isolated mitochondria. Here we detected and characterized mitochondrial DNA damage in isolated rat liver mitochondria exposed to a Fe2+-citrate complex, a small molecular weight complex. Intense DNA fragmentation was induced after the incubation of mitochondria with the iron complex. The detection of 3' phosphoglycolate ends at the mtDNA strand breaks by a 32P-postlabeling assay, suggested the involvement of hydroxyl radical in the DNA fragmentation induced by Fe2+-citrate. Increased levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine also suggested that Fe2+-citrate-induced oxidative stress causes mitochondrial DNA damage. In conclusion, our results show that iron-mediated lipid peroxidation was associated with intense mtDNA damage derived from the direct attack of reactive oxygen species.
Subject: Animals
Dna Damage
Dna, Mitochondrial
Ferrous Compounds
Lipid Peroxidation
Mitochondria, Liver
Mitochondrial Membranes
Mitochondrial Swelling
Rats, Wistar
Citation: Anais Da Academia Brasileira De Ciências. v. 78, n. 3, p. 505-14, 2006-Sep.
Rights: aberto
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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