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Type: Artigo de periódico
Title: Cytotoxic Activity Of Violacein In Human Colon Cancer Cells.
Author: de Carvalho, Daniela D
Costa, Fabio T M
Duran, Nelson
Haun, Marcela
Abstract: Several studies have shown that violacein, a purple pigment extracted from Chromobacterium violaceum, is capable to induce apoptosis in a variety of cancer cells, including those leukemia cell lines. Herein, we examined the effects of violacein on reactive oxygen species (ROS) production during the apoptotic colon cancer cell death. We demonstrate that violacein mediates ROS production followed by activation of Caspase-3, release of cytochrome c, and calcium release to citosol in Caco-2 cells. Moreover, presence of ROS scavengers such as N-acetyl-cysteine (NAC) diminishes ROS cytotoxicity induced by violacein in Caco-2 cells, indicating that violacein mediates cellular critical mechanisms in the triggering of apoptotic tumor cell death. These data also imply that violacein-induced ROS are collectively key mediators of mitochondrial membrane collapse, leading to cytochrome c release, and culminating in tumor apoptosis. Unlike in Caco-2 cells, violacein was incapable of increasing ROS levels in HT29 cells, suggesting the existence of violacein cell-type specific mechanisms. Those findings bring light to the violacein cytotoxic mechanism studies, indicating that oxidative stress play a role in the violacein-induced cytotoxicity.
Subject: Apoptosis
Caco-2 Cells
Caspase 3
Cell Death
Cell Survival
Colonic Neoplasms
Cytochromes C
Enzyme Activation
Ht29 Cells
Indicators And Reagents
Reactive Oxygen Species
Tetrazolium Salts
Citation: Toxicology In Vitro : An International Journal Published In Association With Bibra. v. 20, n. 8, p. 1514-21, 2006-Dec.
Rights: fechado
Identifier DOI: 10.1016/j.tiv.2006.06.007
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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