Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/196811
Type: Artigo de periódico
Title: Role Of Mitochondria In The Immune Response To Cancer: A Central Role For Ca2+.
Author: Degasperi, Giovanna R
Velho, Jesus A
Zecchin, Karina G
Souza, Cláudio T
Velloso, Lício A
Borecký, Jiri
Castilho, Roger F
Vercesi, Anibal E
Abstract: This study demonstrates that Ca2+ stimulates mitochondrial energy metabolism during spleen lymphocyte activation in response to the ascitic Walker 256 tumor in rats. Intracellular Ca2+ concentrations, phosphorylated protein kinase C (pPKC) levels, Bcl-2 protein contents, interleukin-2 (IL-2) levels, mitochondrial uncoupling protein-2 (UCP-2) contents and reactive oxygen species (ROS) were significantly elevated in these activated lymphocytes. Mitochondria of activated lymphocytes exhibited high free Ca2+ concentrations in the matrix and enhanced oligomycin-sensitive oxygen consumption, indicating an increased rate of oxidative phosphorylation. The production of ROS was largely decreased by diphenylene iodinium in the activated lymphocytes, suggesting that NADPH oxidase is the prevalent source of these species. Accumulation of UCP-2 and the anti-apoptotic protein Bcl-2 is probably important to prevent mitochondrial dysfunction and cell death elicited by the sustained high levels of intracellular Ca2+ and ROS and may explain the observed higher resistance from activated lymphocytes against the opening of the mitochondrial membrane permeability pore (MPT). All these changes were blocked by pretreatment of the rats with verapamil, an L-type Ca2+ channel antagonist. These data demonstrate a central role of Ca2+ in the control of mitochondrial bioenergetics in spleen lymphocytes during the immune response to cancer.
Subject: Animals
Calcium
Carcinoma 256, Walker
Cell Death
Energy Metabolism
In Vitro Techniques
Interleukin-2
Ion Channels
Lymphocyte Activation
Lymphocytes
Male
Membrane Transport Proteins
Mitochondria
Mitochondrial Membranes
Mitochondrial Proteins
Nadph Oxidase
Phosphorylation
Protein Kinase C
Proto-oncogene Proteins C-bcl-2
Rats
Rats, Wistar
Reactive Oxygen Species
Spleen
Citation: Journal Of Bioenergetics And Biomembranes. v. 38, n. 1, p. 1-10, 2006-Feb.
Rights: fechado
Identifier DOI: 10.1007/s10863-006-9000-y
Address: http://www.ncbi.nlm.nih.gov/pubmed/16779655
Date Issue: 2006
Appears in Collections:Unicamp - Artigos e Outros Documentos

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