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dc.typeArtigo de periódicopt_BR
dc.titleChromatin Supraorganization, Dna Fragmentation, And Cell Death In Snake Erythrocytes.pt_BR
dc.contributor.authorMiyamoto, Maristelapt_BR
dc.contributor.authorVidal, Benedicto Cpt_BR
dc.contributor.authorMello, Maria Luiza Spt_BR
unicamp.authorMaristela Miyamoto, Department of Cell Biology, Institute of Biology, UNICAMP, 13084-971, Campinas, SP, Brazil.pt_BR C Vidal,pt Luiza S Mello,pt
dc.subjectCell Deathpt_BR
dc.subjectDna Fragmentationpt_BR
dc.subjectIn Situ Nick-end Labelingpt_BR
dc.subjectMicroscopy, Electronpt_BR
dc.subjectRosaniline Dyespt_BR
dc.subjectSouth Americapt_BR
dc.description.abstractIn nucleate erythrocytes of several vertebrate groups, the frequency and intensity of DNA fragmentation associated with programmed cell death vary considerably. Although hemoglobin efficiency may be related to erythrocyte life span, and hemoglobin types and erythrocyte life spans are assumed to vary in reptiles, no data on DNA fragmentation and chromatin organization as related to cell death exist for snakes. In the present study, chromatin supraorganization, DNA fragmentation, and cell death were investigated in four snake species (Crotalus durissus terrificus, Bothrops jararaca, Bothrops alternatus, and Bothrops neuwiedii), which differ in their geographical distribution and habitats, by using image analysis of Feulgen hydrolysis kinetics, the TUNEL assay, single-cell gel electrophoresis, and transmission electron microscopy. Relatively few circulating erythrocytes were found to be simultaneously committed to cell death, although there was some variation among the snake species. Conspicuous nuclear and cytoplasmic organelles suggestive of metabolic activity were seen ultrastructurally in most snake erythrocytes. The DNA of the snake erythrocyte chromatin was much more resistant to Feulgen acid hydrolysis (DNA depurination and breakdown) than that of young adult bullfrog erythrocytes, which had a high frequency and intensity of DNA fragmentation. Of the species studied, B. neuwiedii and C. d. terrificus showed the greatest resistance to Feulgen acid hydrolysis and to the DNA fragmentation, revealed by the TUNEL assay. Although B. neuwiedii also showed the lowest frequency of cells with more damaged DNA in the single-cell gel electrophoresis assay, C. d. terrificus had the highest frequency of damaged cells, possibly because of the abundance of alkaline-sensitive DNA sites. The results for DNA fragmentation and cell death in erythrocytes of B. jararaca and B. alternatus generally differed from those for C. d. terrificus and B. neuwiedii and may reflect differences in the biology of these species selected under different geographical habitats. The differences in erythrocyte cell biology reported here may be related to hemoglobin variants selected in the mentioned snake species and that would lead the cells to different resistances to unfavorable environmental conditions.en
dc.relation.ispartofBiochemistry And Cell Biology = Biochimie Et Biologie Cellulairept_BR
dc.relation.ispartofabbreviationBiochem. Cell Biol.pt_BR
dc.identifier.citationBiochemistry And Cell Biology = Biochimie Et Biologie Cellulaire. v. 83, n. 1, p. 15-27, 2005-Feb.pt_BR
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