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Type: Artigo de periódico
Title: High Performance Liquid Chromatography Analysis Of A 4-anilinoquinazoline Derivative (pd153035), A Specific Inhibitor Of The Epidermal Growth Factor Receptor Tyrosine Kinase, In Rat Plasma.
Author: Rocco, Silvana Aparecida
Velho, Jesus Antonio
Marin, Rodrigo Miguel
de Arruda Rolim Filho, Luís
Vercesi, Anibal Eugênio
Rittner, Roberto
Franchini, Kleber Gomes
Abstract: The quinazoline derivative, 4-N-(3'-bromo-phenyl)amino-6,7-dimethoxyquinazoline (PD153035), has recently been identified as a potential drug for the treatment of proliferative disease. Here, we report a sensitive high performance liquid chromatography (HPLC)-based quantitative detection method for measurement of PD153035 levels in rat plasma. Sample pretreatment involved a two-step extraction with chloroform. The analytes were separated on a column packed with OmniSpher C18 material and eluted with acetonitrile-0.1 M ammonium acetate, pH 7.2 (70:30, v/v). The column effluent was monitored by UV detection at 330 nm. A linear response was achieved over the concentration range 0.50-100.00 microM using multilevel calibration with an internal standard. The analytical method inter- and intra-run accuracy and precision were better than +/-15%. The lower limit of quantification was 0.50 microM. The method has been applied to study the preclinical pharmacokinetics of this compound in rats.
Subject: Animals
Chromatography, High Pressure Liquid
Enzyme Inhibitors
Rats, Wistar
Receptor, Epidermal Growth Factor
Reference Standards
Reproducibility Of Results
Sensitivity And Specificity
Spectrophotometry, Ultraviolet
Citation: Journal Of Chromatography. B, Analytical Technologies In The Biomedical And Life Sciences. v. 817, n. 2, p. 297-302, 2005-Mar.
Rights: fechado
Identifier DOI: 10.1016/j.jchromb.2004.12.021
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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