Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Increased Expression Of Advanced Glycation End-products And Their Receptor, And Activation Of Nuclear Factor Kappa-b In Lacrimal Glands Of Diabetic Rats.
Author: Alves, M
Calegari, V C
Cunha, D A
Saad, M J A
Velloso, L A
Rocha, E M
Abstract: To assess the involvement of the AGE-specific receptor (AGER, also known as RAGE) axis and nuclear factor kappa-B (NFKB, also known as NF-kappaB) activation in the development of lacrimal gland and tear film dysfunction in diabetes, the present study evaluated: (1) lacrimal gland and tear film alterations in diabetic rats; and (2) the expression of AGE, AGER and NFKB in ocular tissues of normoglycaemic and diabetic rats. Diabetes was induced in male Wistar rats with intravenous streptozotocin. Tear secretion parameters were measured and NFKB expression was evaluated in lacrimal glands of control and diabetic rats by western blot. Immunohistochemistry with confocal microscopy was used to assess AGE, AGER and NFKB expression in lacrimal glands of both groups. Lacrimal gland weight and tear film volume were lower in diabetic than in control rats (p=0.01 and 0.02, respectively). IL1B and TNF concentrations in tears were higher in diabetic than in control rats (p=0.007 and 0.02, respectively). NFKB protein was identified in rat cornea, conjunctiva and lacrimal glands. AGE, AGER and NFKB expression were greater in lacrimal glands of diabetic than in those of control rats. Diabetes induces significant alterations in rat lacrimal gland structure and secretion. The higher expression of AGE, AGER and NFKB in lacrimal glands of diabetic rats suggests that these factors are involved in signalling and in subsequent inflammatory alterations related to dry eye in diabetes mellitus.
Subject: Animals
Blotting, Western
Diabetes Mellitus, Experimental
Dry Eye Syndromes
Gene Expression
Glycosylation End Products, Advanced
Lacrimal Apparatus
Nf-kappa B
Rats, Wistar
Receptors, Immunologic
Tumor Necrosis Factor-alpha
Citation: Diabetologia. v. 48, n. 12, p. 2675-81, 2005-Dec.
Rights: fechado
Identifier DOI: 10.1007/s00125-005-0010-9
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_16283249.pdf273.56 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.