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dc.typeArtigo de periódicopt_BR
dc.titleCadmium Chloride-induced Oxidative Stress In Skeletal Muscle Cells In Vitro.pt_BR
dc.contributor.authorYano, Claudia Lumypt_BR
dc.contributor.authorMarcondes, Maria Cristina Cintra Gomespt_BR
unicamp.authorClaudia Lumy Yano, Departamento de Fisiologia e Biofísica e Departamento de Biologia Celular, Instituto de Biologia, Universidade Estadual de Campinas UNICAMP, CP 6109, 13083-970, Campinas, São Paulo, SP, Brazil.pt_BR Cristina Cintra Gomes Marcondes,pt
dc.subjectCadmium Chloridept_BR
dc.subjectCell Adhesionpt_BR
dc.subjectCell Deathpt_BR
dc.subjectCell Differentiationpt_BR
dc.subjectCell Linept_BR
dc.subjectCell Survivalpt_BR
dc.subjectDose-response Relationship, Drugpt_BR
dc.subjectGlutathione Transferasept_BR
dc.subjectIn Vitro Techniquespt_BR
dc.subjectLipid Peroxidationpt_BR
dc.subjectMicroscopy, Electron, Scanningpt_BR
dc.subjectMuscle, Skeletalpt_BR
dc.subjectOxidative Stresspt_BR
dc.subjectSignal Transductionpt_BR
dc.subjectTetrazolium Saltspt_BR
dc.subjectTime Factorspt_BR
dc.description.abstractThe effects of cadmium chloride (CdCl(2)) on oxidative stress in the skeletal muscle cell line C(2)C(12) were investigated. Myoblast cells that differentiated into myotubes were treated with CdCl(2) (1, 3, 5, 7.5, 10, and 12.5 microM) for 24, 48, and 72 h. Subsequent assay of cell homogenates for MTT (3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide) reduction, neutral red uptake and nucleic acid content showed that cadmium was toxic to C(2)C(12) cells in a concentration-dependent manner. Glutathione-S-transferase activity (nmol microg of protein(-1) min(-1)) was increased with 1 and 3 microM CdCl(2) (36.9 +/- 5.6 and 32.1 +/- 6.0, respectively) compared to control cells (21.8 +/- 1.5), but decreased at higher concentrations (7.5 microM = 15.9 +/- 3.3, 10 microM = 15.9 +/- 4.6, and 12.5 microM = 10.5 +/- 2.8). An increase in malondialdehyde content (nmol microg of protein(-1)), especially at high CdCl(2) concentrations (control = 7.3 +/- 0.5; CdCl(2): 7.5 microM = 11.2 +/- 3.1, 10 microM = 14.6 +/- 3.8, and 12.5 microM = 20.5 +/- 6.5) indicated that there was enhanced lipid peroxidation. Light and scanning electron microscopy showed that there was a concentration-dependent loss of adherent cells and the formation of vesicles indicative of cell death. These results indicated that CdCl(2) increased oxidative stress in C(2)C(12) cells, and this stress probably compromised cell adhesion and the cellular antioxidant defense mechanisms.en
dc.relation.ispartofFree Radical Biology & Medicinept_BR
dc.relation.ispartofabbreviationFree Radic. Biol. Med.pt_BR
dc.identifier.citationFree Radical Biology & Medicine. v. 39, n. 10, p. 1378-84, 2005-Nov.pt_BR
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