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Type: Artigo de periódico
Title: Cadmium Chloride-induced Oxidative Stress In Skeletal Muscle Cells In Vitro.
Author: Yano, Claudia Lumy
Marcondes, Maria Cristina Cintra Gomes
Abstract: The effects of cadmium chloride (CdCl(2)) on oxidative stress in the skeletal muscle cell line C(2)C(12) were investigated. Myoblast cells that differentiated into myotubes were treated with CdCl(2) (1, 3, 5, 7.5, 10, and 12.5 microM) for 24, 48, and 72 h. Subsequent assay of cell homogenates for MTT (3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide) reduction, neutral red uptake and nucleic acid content showed that cadmium was toxic to C(2)C(12) cells in a concentration-dependent manner. Glutathione-S-transferase activity (nmol microg of protein(-1) min(-1)) was increased with 1 and 3 microM CdCl(2) (36.9 +/- 5.6 and 32.1 +/- 6.0, respectively) compared to control cells (21.8 +/- 1.5), but decreased at higher concentrations (7.5 microM = 15.9 +/- 3.3, 10 microM = 15.9 +/- 4.6, and 12.5 microM = 10.5 +/- 2.8). An increase in malondialdehyde content (nmol microg of protein(-1)), especially at high CdCl(2) concentrations (control = 7.3 +/- 0.5; CdCl(2): 7.5 microM = 11.2 +/- 3.1, 10 microM = 14.6 +/- 3.8, and 12.5 microM = 20.5 +/- 6.5) indicated that there was enhanced lipid peroxidation. Light and scanning electron microscopy showed that there was a concentration-dependent loss of adherent cells and the formation of vesicles indicative of cell death. These results indicated that CdCl(2) increased oxidative stress in C(2)C(12) cells, and this stress probably compromised cell adhesion and the cellular antioxidant defense mechanisms.
Subject: Animals
Cadmium Chloride
Cell Adhesion
Cell Death
Cell Differentiation
Cell Line
Cell Survival
Dose-response Relationship, Drug
Glutathione Transferase
In Vitro Techniques
Lipid Peroxidation
Microscopy, Electron, Scanning
Muscle, Skeletal
Oxidative Stress
Signal Transduction
Tetrazolium Salts
Time Factors
Citation: Free Radical Biology & Medicine. v. 39, n. 10, p. 1378-84, 2005-Nov.
Rights: fechado
Identifier DOI: 10.1016/j.freeradbiomed.2005.07.001
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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