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Type: Artigo de periódico
Title: Cross-neutralization Of The Neurotoxicity Of Crotalus Durissus Terrificus And Bothrops Jararacussu Venoms By Antisera Against Crotoxin And Phospholipase A2 From Crotalus Durissus Cascavella Venom.
Author: Beghini, Daniela G
da Cruz-Höfling, Maria Alice
Randazzo-Moura, Priscila
Rodrigues-Simioni, Léa
Novello, José Camilo
Hyslop, Stephen
Marangoni, Sérgio
Abstract: We have previously demonstrated that rabbit antisera raised against crotoxin from Crotalus durissus cascavella venom (cdc-crotoxin) and its PLA2 (cdc-PLA2) neutralized the neurotoxicity of this venom and its crotoxin. In this study, we examined the ability of these antisera to neutralize the neurotoxicity of Crotalus durissus terrificus and Bothrops jararacussu venoms and their major toxins, cdt-crotoxin and bothropstoxin-I (BthTX-I), respectively, in mouse isolated phrenic nerve-diaphragm preparations. Immunoblotting showed that antiserum to cdc-crotoxin recognized cdt-crotoxin and BthTX-I, while antiserum to cdc-PLA2 recognized cdt-PLA2 and BthTX-I. ELISA corroborated this cross-reactivity. Antiserum to cdc-crotoxin prevented the neuromuscular blockade caused by C. d. terrificus venom and its crotoxin at a venom/crotoxin:antiserum ratio of 1:3. Antiserum to cdc-PLA2 also neutralized the neuromuscular blockade caused by C. d. terrificus venom or its crotoxin at venom or toxin:antiserum ratios of 1:3 and 1:1, respectively. The neuromuscular blockade caused by B. jararacussu venom and BthTX-I was also neutralized by the antisera to cdc-crotoxin and cdc-PLA2 at a venom/toxin:antiserum ratio of 1:10 for both. Commercial equine antivenom raised against C. d. terrificus venom was effective in preventing the neuromuscular blockade typical of B. jararacussu venom (venom:antivenom ratio of 1:2), whereas for BthTX-I the ratio was 1:10. These results show that antiserum produced against PLA2, the major toxin in C. durissus cascavella venom, efficiently neutralized the neurotoxicity of C. d. terrificus and B. jararacussu venoms and their PLA2 toxins.
Subject: Analysis Of Variance
Chromatography, Affinity
Cross Reactions
Crotalid Venoms
Enzyme-linked Immunosorbent Assay
Immune Sera
Neuromuscular Junction
Neutralization Tests
Phospholipases A
Phospholipases A2
Citation: Toxicon : Official Journal Of The International Society On Toxinology. v. 46, n. 6, p. 604-11, 2005-Nov.
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2005.06.018
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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