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Type: Artigo de periódico
Title: Nitrite As The Major Source Of Nitric Oxide Production By Arabidopsis Thaliana In Response To Pseudomonas Syringae.
Author: Modolo, Luzia V
Augusto, Ohara
Almeida, Irene M G
Magalhaes, Jose R
Salgado, Ione
Abstract: The origin of nitric oxide (*NO) in plants is unclear and an *NO synthase (NOS)-like enzyme and nitrate reductase (NR) are claimed as potential sources. Here we used wild-type and NR-defective double mutant plants to investigate *NO production in Arabidopsis thaliana in response to Pseudomonas syringae pv maculicola. NOS activity increased substantially in leaves inoculated with P. syringae. However, electron paramagnetic resonance experiments showed a much higher *NO formation that was dependent on nitrite and mitochondrial electron transport rather than on arginine or nitrate. Overall, these results indicate that NOS, NR and a mitochondrial-dependent nitrite-reducing activity cooperate to produce *NO during A. thaliana-P. syringae interaction.
Subject: Arabidopsis
Electron Spin Resonance Spectroscopy
Nitrate Reductase
Nitrate Reductases
Nitric Oxide
Nitric Oxide Synthase
Plant Leaves
Pseudomonas Syringae
Citation: Febs Letters. v. 579, n. 17, p. 3814-20, 2005-Jul.
Rights: fechado
Identifier DOI: 10.1016/j.febslet.2005.05.078
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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