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Type: Artigo de periódico
Title: Porphyromonas Gingivalis, Porphyromonas Endodontalis, Prevotella Intermedia And Prevotella Nigrescens In Endodontic Lesions Detected By Culture And By Pcr.
Author: Gomes, B P F A
Jacinto, R C
Pinheiro, E T
Sousa, E L R
Zaia, A A
Ferraz, C C R
Souza-Filho, F J
Abstract: he aim of this study was to investigate the presence of four black-pigmented bacteria, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens, in endodontic infections by culture and polymerase chain reaction (PCR) analyses. Microbial samples were obtained from 50 teeth with untreated necrotic pulps (primary infection) and from 50 teeth with failing endodontic treatment (secondary infection). Microbiological strict anaerobic techniques were used for serial dilution, plating, incubation, and identification. For PCR detection, the samples were analyzed using species-specific primers of 16S rDNA and the downstream intergenic spacer region. Culture and PCR detected the test species in 13/100 and 50/100 of the study teeth, respectively. The organisms were cultured from 11/50 (22%) of primarily infected root canal samples and from 2/50 (4%) of secondary root canal samples. PCR detection identified the target species in 32/50 (64%) and 18/50 (36%) of primary and secondary infections, respectively. P. gingivalis was rarely isolated by culture methods (1%), but was the most frequently identified test species by PCR (38%). Similarly, P. endodontalis was not recovered by culture from any tooth studied, but was detected by PCR in 25% of the sampled teeth. PCR-based identification also showed higher detection rates of P. intermedia (33%) and P. nigrescens (22%) than culture (13%). In conclusion, P. gingivalis, P. endodontalis, P. intermedia, and P. nigrescens were identified more frequently in teeth with necrotic pulp than in teeth with failing endodontic treatment. Also, a higher frequency of black-pigmented species was detected by PCR than by culture.
Subject: Bacterial Typing Techniques
Bacteroidaceae Infections
Colony Count, Microbial
Dna, Bacterial
Dental Pulp Necrosis
Dental Restoration Failure
Polymerase Chain Reaction
Porphyromonas Endodontalis
Porphyromonas Gingivalis
Prevotella Intermedia
Prevotella Nigrescens
Citation: Oral Microbiology And Immunology. v. 20, n. 4, p. 211-5, 2005-Aug.
Rights: fechado
Identifier DOI: 10.1111/j.1399-302X.2005.00214.x
Date Issue: 2005
Appears in Collections:Unicamp - Artigos e Outros Documentos

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