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Type: Artigo de periódico
Title: Ibuprofen-induced Walker 256 Tumor Cell Death: Cytochrome C Release From Functional Mitochondria And Enhancement By Calcineurin Inhibition.
Author: Campos, Claudia B L
Degasperi, Giovanna R
Pacífico, Denise S
Alberici, Luciane C
Carreira, Raquel S
Guimarães, Fernando
Castilho, Roger F
Vercesi, Anibal E
Abstract: The participation of mitochondria in the mechanism of tumor cell death induced by non-steroid anti-inflammatory drugs is uncertain. Here we show that ibuprofen induces death of Walker 256 tumor cells independently on mitochondrial depolarization as estimated by flow cytometry using DioC(6)(3). Oligomycin increased mitochondrial transmembrane potential in both ibuprofen-treated and non-treated cells, indicating that ATP synthesis was sustained during cell death. Cyclosporin A, but not bongkrekic acid, both mitochondrial permeability transition inhibitors, increased the percentage of cell death in the presence of ibuprofen. FK506, a calcineurin inhibitor like cyclosporin A, also increased ibuprofen-induced cell death. Moreover, we showed that cytochrome c was released during ibuprofen-induced cell death. In conclusion, death of Walker 256 tumor cells induced by ibuprofen does not impair mitochondrial function, involves cytochrome c release and is accompanied by a rescue pathway via calcineurin activation.
Subject: Adenosine Triphosphate
Anti-inflammatory Agents, Non-steroidal
Calcineurin Inhibitors
Cell Death
Cytochromes C
Ion Channels
Membrane Potentials
Mitochondrial Membrane Transport Proteins
Tumor Cells, Cultured
Citation: Biochemical Pharmacology. v. 68, n. 11, p. 2197-206, 2004-Dec.
Rights: fechado
Identifier DOI: 10.1016/j.bcp.2004.08.006
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

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