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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleIn Vitro Analysis Of Anionic Collagen Scaffolds For Bone Repair.pt_BR
dc.contributor.authorMoreira, Patricia Lpt_BR
dc.contributor.authorAn, Yuehuei Hpt_BR
dc.contributor.authorSantos, Arnaldo Rodriguespt_BR
dc.contributor.authorGenari, Selma Candeláriapt_BR
unicamp.authorPatricia L Moreira, Department of Cellular Biology, State University of Campinas, PO Box 6109, São Paulo 13084-971, Brazil. plm@unicamp.brpt_BR
unicamp.author.externalYuehuei H An,pt
unicamp.author.externalArnaldo Rodrigues Santos,pt
unicamp.author.externalSelma Candelária Genari,pt
dc.subjectAlkaliespt_BR
dc.subjectAlkaline Phosphatasept_BR
dc.subjectAnimalspt_BR
dc.subjectBone And Bonespt_BR
dc.subjectCattlept_BR
dc.subjectCell Adhesionpt_BR
dc.subjectCell Linept_BR
dc.subjectChondroitin Sulfatespt_BR
dc.subjectCollagenpt_BR
dc.subjectColoring Agentspt_BR
dc.subjectFractures, Bonept_BR
dc.subjectHydrolysispt_BR
dc.subjectIn Vitro Techniquespt_BR
dc.subjectOsteoblastspt_BR
dc.description.abstractCollagen has been extensively described as a beneficial material in bone tissue engineering due to its biocompatibility, biodegradability, low antigenicity, and high tensile strength. However, collagen scaffolds in their pure form have some drawbacks and improvements in the physical, chemical, and biologic properties of collagen are necessary to overcome those inadequacies. Recently, the selective hydrolysis of carboxyamides of asparagine and glutamine residues of collagen has been employed to increase the number of negative sites and enhance the piezoelectric properties of collagen. Anionic collagen scaffolds were prepared by use of a hydrolysis treatment for either 24 h [bovine pericardium (BP 24)] or 48 h (BP 48). Bovine osteoblasts were cultured on them and on native matrices to understand the cellular interactions responsible for the good osteoconductivity and biocompatibility reported with in vivo tests. Based on the data obtained on cell adhesion, alkaline phosphatase (ALP) and extracellular matrix macromolecule production, and cellular proliferation through histological analysis, we may conclude that the materials tested reveal sufficient biocompatibility level for bone repair. Further, the evidence of some connection between ALP activity and the mineralization process should be emphasized. BP 48 presented the most promising results stimulating in vitro mineralization, ALP production, and possible osteoblast differentiation.en
dc.relation.ispartofJournal Of Biomedical Materials Research. Part B, Applied Biomaterialspt_BR
dc.relation.ispartofabbreviationJ. Biomed. Mater. Res. Part B Appl. Biomater.pt_BR
dc.date.issued2004-Novpt_BR
dc.identifier.citationJournal Of Biomedical Materials Research. Part B, Applied Biomaterials. v. 71, n. 2, p. 229-37, 2004-Nov.pt_BR
dc.language.isoengpt_BR
dc.description.volume71pt_BR
dc.description.firstpage229-37pt_BR
dc.rightsfechadopt_BR
dc.rights.holder(c) 2004 Wiley Periodicals, Inc.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1552-4973pt_BR
dc.identifier.doi10.1002/jbm.b.30026pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/15386402pt_BR
dc.date.available2015-11-27T12:58:28Z-
dc.date.accessioned2015-11-27T12:58:28Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:58:28Z (GMT). No. of bitstreams: 1 pmed_15386402.pdf: 425123 bytes, checksum: 8fe44abcef1552490692a6b6a7108c20 (MD5) Previous issue date: 2004en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195957-
dc.identifier.idPubmed15386402pt_BR
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