Please use this identifier to cite or link to this item:
Type: Artigo de periódico
Title: Decreased Insulin Secretion In Islets From Rats Fed A Low Protein Diet Is Associated With A Reduced Pkaalpha Expression.
Author: Ferreira, Fabiano
Barbosa, Helena C L
Stoppiglia, Luiz Fabrizio
Delghingaro-Augusto, Viviane
Pereira, Eliana A
Boschero, Antonio C
Carneiro, Everardo M
Abstract: A low protein diet has been shown to affect the amount and activity of several enzymes and to decrease insulin secretion by islets isolated from rats fed such a diet. To understand the mechanisms involved in this phenomenon, we investigated the effects of forskolin, a stimulator of adenylyl cyclase, on insulin secretion by pancreatic islets from rats fed a normal (17%; NP) or low (6%; LP) protein diet for 8 wk. Isolated islets were incubated for 1 h in Krebs-bicarbonate solution containing 8.3 mmol glucose/L, with or without 10 micromol forskolin/L. The forskolin-induced insulin secretion was higher in islets from NP rats than in those from LP rats (P<0.05). Western blotting revealed that the amount of the alpha catalytic subunit of protein kinase A (PKAalpha) was 35% lower in islets from LP rats than in islets from NP rats (P<0.05). Moreover, PKAalpha mRNA expression was reduced by 30% in islets from LP rats (P<0.05). Our results indicated a possible relationship between a low protein diet and a reduction in PKAalpha expression. These alterations in PKAalpha may be responsible in part for the decreased insulin secretion by islets from rats fed a low protein diet.
Subject: Adenylate Cyclase
Blotting, Western
Cyclic Amp-dependent Protein Kinases
Diet, Protein-restricted
Gene Expression
Islets Of Langerhans
Rna, Messenger
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Citation: The Journal Of Nutrition. v. 134, n. 1, p. 63-7, 2004-Jan.
Rights: fechado
Date Issue: 2004
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_14704294.pdf260.56 kBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.