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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleDna Content, Chromatin Texture And Nuclear Morphology In Benzo[a]pyrene-transformed Human Breast Epithelial Cells After Microcell-mediated Transfer Of Chromosomes 11 And 17.pt_BR
dc.contributor.authorMello, Maria Luiza Spt_BR
dc.contributor.authorde Campos Vidal, Benedictopt_BR
dc.contributor.authorLareef, Mohamed Hpt_BR
dc.contributor.authorHu, Yun Fpt_BR
dc.contributor.authorYang, Xiaoqipt_BR
dc.contributor.authorRusso, Josept_BR
unicamp.authorMaria Luiza S Mello, Department of Cell Biology, Institute of Biology, UNICAMP, Campinas, São Paolo, Brazil. mlsmello@obelix.unicamp.brpt_BR
unicamp.author.externalBenedicto de Campos Vidal,pt
unicamp.author.externalMohamed H Lareef,pt
unicamp.author.externalYun F Hu,pt
unicamp.author.externalXiaoqi Yang,pt
unicamp.author.externalJose Russo,pt
dc.subjectBenzo(a)pyrenept_BR
dc.subjectBreastpt_BR
dc.subjectCell Line, Transformedpt_BR
dc.subjectCell Nucleuspt_BR
dc.subjectCell Transformation, Neoplasticpt_BR
dc.subjectChromatinpt_BR
dc.subjectChromosomes, Human, Pair 11pt_BR
dc.subjectChromosomes, Human, Pair 17pt_BR
dc.subjectDnapt_BR
dc.subjectEpithelial Cellspt_BR
dc.subjectFemalept_BR
dc.subjectGene Transfer Techniquespt_BR
dc.subjectHumanspt_BR
dc.subjectImage Cytometrypt_BR
dc.subjectMicroscopy, Videopt_BR
dc.description.abstractA relation between the changes in DNA content and chromatin supra-organization and the expression of gradual steps of tumorigenesis has been assessed by image analysis in human breast epithelial cells (MCF-10F) treated with benzo[a]pyrene (BP) (cell lines BP1, BP1-E, BP1-Tras, and others). Because abnormal chromosomes 11 and 17 have been associated with neoplastic progression in BP-transformed MCF-10F cells, image analysis of Feulgen-stained tumorigenic BP1-E cells with the microcell-mediated chromosome transfer of normal chromosomes 11 and 17 was carried out. A tendency of DNA amount distribution and nuclear size restoration to values typical of non-transformed MCF-10F cells was demonstrated, especially after the transfer of chromosome 17. No reversion in chromatin texture was found after the transfer of chromosome 11 or 17. Although the presence of a normal chromosome 17 should be considered among the necessary steps for tumorigenic human breast epithelial cells to recover their normality, a more complex genome balance is required for the entire nuclear chromatin of these cells to recover its totally normal supra-organization and expression.en
dc.relation.ispartofCytometry. Part A : The Journal Of The International Society For Analytical Cytologypt_BR
dc.relation.ispartofabbreviationCytometry Apt_BR
dc.date.issued2003-Aprpt_BR
dc.identifier.citationCytometry. Part A : The Journal Of The International Society For Analytical Cytology. v. 52, n. 2, p. 70-6, 2003-Apr.pt_BR
dc.language.isoengpt_BR
dc.description.volume52pt_BR
dc.description.firstpage70-6pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright 2003 Wiley-Liss, Inc.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1552-4922pt_BR
dc.identifier.doi10.1002/cyto.a.10029pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/12655650pt_BR
dc.date.available2015-11-27T12:52:45Z-
dc.date.accessioned2015-11-27T12:52:45Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:52:45Z (GMT). No. of bitstreams: 1 pmed_12655650.pdf: 448855 bytes, checksum: ba3fb25a55e83b477d994a4d05c8b50e (MD5) Previous issue date: 2003en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/195703-
dc.identifier.idPubmed12655650pt_BR
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