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Type: Artigo de periódico
Title: Participation Of Na(+) Channels In The Potentiation By Tityus Serrulatus Alpha-toxin Tstx-v Of Glucose-induced Electrical Activity And Insulin Secretion In Rodent Islet Beta-cells.
Author: Gonçalves, Antonio A
Toyama, Marcos H
Carneiro, Everardo M
Marangoni, Sergio
Arantes, Eliana C
Giglio, José R
Boschero, Antonio C
Abstract: The effects of TsTx-V, an alpha-toxin isolated from Tityus serrulatus venom, on electrical activity and insulin secretion by rodent pancreatic islet cells were studied. TsTx-V (5.6 microg/ml) depolarized mouse pancreatic beta-cells, diminished the membrane input resistance and increased the duration of the active phase of glucose-induced electrical activity. Similar results were observed with the Na(+) channel agonist veratridine (110 microM). Both agents potentiated glucose (8.3 mM)-induced insulin secretion in rat islet. In the presence of TsTx-V or veratridine, insulin secretion increased 2- and 1.4-fold over basal values, respectively (P<0.001). The Na(+) channel antagonist tetrodotoxin (6 microM) significantly decreased glucose- and TsTx-V-induced insulin secretion (P<0.001). TsTx-V also stimulated insulin secretion at low glucose concentrations (2.8 mM) whereas the beta-toxin, Ts-gamma (gamma toxin), also obtained from Tityus serrulatus venom, significantly reduced TsTx-V-induced secretion at sub- and suprathreshold concentrations of glucose. These results are consistent with a model whereby Na(+) channels participate in glucose-induced electrical activity. Alteration in the activity of these channels changes the length of time during which the beta-cell depolarizes, thereby altering the secretory behavior of the cell. The construction of a three-dimensional model for TsTx-V revealed a conserved core containing an alpha-helix and three beta-strands, with minor differences when compared with toxins from other scorpion venoms.
Subject: Animals
Electric Conductivity
Islets Of Langerhans
Molecular Conformation
Scorpion Venoms
Sodium Channel Agonists
Sodium Channels
Citation: Toxicon : Official Journal Of The International Society On Toxinology. v. 41, n. 8, p. 1039-45, 2003-Jun.
Rights: fechado
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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