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Type: Artigo de periódico
Title: Addition Of Exogenous Cytokines In Mixed Lymphocyte Culture For Selecting Related Donors For Bone Marrow Transplantation.
Author: Visentainer, Jeane Eliete Laguila
Lieber, Sofia Rocha
Persoli, L gia Beatriz Lopes
Vigorito, Afonso Celso
Aranha, Francisco Jos Penteado
Souza, C rmino Antonio de
Abstract: Mixed lymphocyte culturing has led to conflicting opinions regarding the selection of donors for bone marrow transplantation. The association between a positive mixed lymphocyte culture and the development of graft-versus-host disease (GVHD) is unclear. The use of exogenous cytokines in mixed lymphocyte cultures could be an alternative for increasing the sensitivity of culture tests. To increase the sensitivity of mixed lymphocyte cultures between donor and recipient human leukocyte antigen (HLA) identical siblings, using exogenous cytokines, in order to predict post-transplantation GVHD and/or rejection. Prospective study. Bone Marrow Transplantation Unit, Universidade Estadual de Campinas. Seventeen patients with hematological malignancies and their respective donors selected for bone marrow transplantation procedures. Standard and modified mixed lymphocyte culturing by cytokine supplementation was carried out using donor and recipient cells typed for HLA. Autologous and allogenic responses in mixed lymphocyte cultures after the addition of IL-4 or IL-2. In comparison with the standard method, average responses in the modified mixed lymphocyte cultures increased by a factor of 2.0 using IL-4 (p < 0.001) and 6.4 using IL-2 (p < 0.001), for autologous donor culture responses. For donor-versus-recipient culture responses, the increase was by a factor of 1.9 using IL-4 (p < 0.001) and 4.1 using IL-2 (p < 0.001). For donor-versus-unrelated culture responses, no significant increase was observed using IL-4, and a mean response inhibition of 20% was observed using IL-2 (p < 0.001). Neither of the cytokines produced a significant difference in the unrelated control versus recipient cell responses. IL-4 supplementation was the best for increasing the mixed lymphocyte culture sensitivity. However, IL-4 also increased autologous responses, albeit less intensively than IL-2. Thus, with this loss of specificity we believe that it is not worth modifying the traditional mixed lymphocyte culture method, even with IL-4 addition.
Subject: Adjuvants, Immunologic
Bone Marrow Transplantation
Graft Vs Host Disease
Lymphocyte Culture Test, Mixed
Prospective Studies
Sensitivity And Specificity
Citation: São Paulo Medical Journal = Revista Paulista De Medicina. v. 120, n. 6, p. 175-9, 2002-Nov.
Rights: aberto
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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