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Type: Artigo de periódico
Title: Nitric Oxide Has A Role In Regulating Vla-4-integrin Expression On The Human Neutrophil Cell Surface.
Author: Conran, Nicola
Gambero, Alessandra
Ferreira, Heloisa H A
Antunes, Edson
de Nucci, Gilberto
Abstract: Recent research demonstrates that the beta1 integrins may be involved in neutrophil migration. Here, we investigate the role of nitric oxide in the expression and function of the very late antigen-4 (VLA-4) and Mac-1 integrins on human neutrophils. Human blood neutrophils were treated with N(omega)-nitro-L-arginine methyl ester (L-NAME) and their adhesion to fibronectin (FN) and serum observed. Adhesion of neutrophils to FN and serum increased significantly following incubation with 0.1mM L-NAME by 65.5 and 44.6%, respectively. Increased adhesions to FN and serum were abolished by a VLA-4-specific monoclonal antibody, HP2/1, and a Mac-1-specific monoclonal antibody, ICRF 44, respectively. The microfilament- and microtubule-depolymerizing agents, dihydrochalasin B and nocodazole, were also able to reverse L-NAME-induced adhesion to both FN and serum. L-NAME induced a discrete increase in the expression of CD49d (VLA-4, 25.3+/-4.8%), but not CD11b, on the neutrophil cell surface, as detected by flow cytometry. Results indicate that NO has a role in regulating VLA-4 and Mac-1 function on the human neutrophil cell surface and that this modulation in integrin function is accompanied by cytoskeletal rearrangements and changes in the ability of the neutrophil to adhere to the extracellular matrix.
Subject: Antibodies, Monoclonal
Cell Adhesion
Cell Adhesion Molecules
Cells, Cultured
Drug Interactions
Enzyme Inhibitors
Guanylate Cyclase
Integrin Alpha4beta1
Macrophage-1 Antigen
Ng-nitroarginine Methyl Ester
Nitric Oxide
Citation: Biochemical Pharmacology. v. 66, n. 1, p. 43-50, 2003-Jul.
Rights: fechado
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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