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Type: Artigo de periódico
Title: Genotyping Of Human Cytomegalovirus Using Non-radioactive Single-strand Conformation Polymorphism (sscp) Analysis.
Author: de Albuquerque, Dulcinéia Martins
Costa, Sandra Cecília Botelho
Abstract: Genetic variation in the glycoprotein B (gB) gene may play a role in human cytomegaloviruses (HCMVs) pathogenesis. Using restriction analysis of the gB gene product (PCR-RFLP), amplified by the nested polymerase chain reaction, the HCMV strains can be compared and classified into at least four HCMV groups. PCR single-strand conformation polymorphism (PCR-SSCP) is one of the techniques used to identify a mutant sequence or a polymorphism in a known gene. SSCP analysis has the advantage over RFLP analysis on detection of DNA polymorphisms and point mutations at a variety of positions in DNA fragments. However, the original SSCP protocols using the incorporation of radioactive label and polyacrylamide gel electrophoresis for detection are labour intensive and time-consuming. A simplified SSCP protocol is described to identify HCMV strains and the gB genotype, allowing the detection of sequence variations not residing in the endonuclease recognition sites.
Subject: Bone Marrow Transplantation
Cytomegalovirus Infections
Genetic Variation
Point Mutation
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single-stranded Conformational
Reproducibility Of Results
Sensitivity And Specificity
Viral Envelope Proteins
Citation: Journal Of Virological Methods. v. 110, n. 1, p. 25-8, 2003-Jun.
Rights: fechado
Date Issue: 2003
Appears in Collections:Unicamp - Artigos e Outros Documentos

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