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Type: Artigo de periódico
Title: Expression Of A Thioredoxin Peroxidase In Insulin-producing Cells.
Author: Boschero, A C
Stoppiglia, L F
Collares-Buzato, C B
Bosqueiro, J R
Delghingaro-Augusto, V
Leite, A
Carvalho, C P F
Netto, L E S
Carneiro, E M
Abstract: The presence of thioredoxin peroxidase (TPx), also known as thiol specific antioxidant (TSA), was investigated in neonatal and adult rat islets, and in the beta-cell line HIT-T15. Western blotting of extracts from neonatal and adult pancreatic islets and from the tumoral cell line HIT-T15 revealed the presence of a 25 kDa protein that comigrated with purified yeast TPx. Endocrine pancreatic TPx accounted for approximately 0.01% of the total protein content. Treatment with H2O2 for 3 h increased the expression of TPx in HIT-T15 cells. The distribution of TPx throughout the islet cells was confirmed by immunocytochemistry. Since pancreatic beta-cells possess a weak antioxidant enzyme defense system, especially with regard to hydrogen peroxidase-decomposing enzymes, the presence of a TPx analog in islets suggests that this enzyme may play a role in protecting pancreatic cells against reactive oxygen species.
Subject: Aging
Animals, Newborn
Cells, Cultured
Islets Of Langerhans
Neoplasm Proteins
Oxidative Stress
Pancreatic Neoplasms
Rats, Wistar
Tumor Cells, Cultured
Citation: Diabetes & Metabolism. v. 28, n. 6 Pt 2, p. 3S25-8; discussion 3S108-12, 2002-Dec.
Rights: fechado
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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