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dc.typeArtigo de periódicopt_BR
dc.titleDot-enzyme Linked Immunosorbent Assay As An Alternative Technique For The Detection Of Bovine Respiratory Syncytial Virus (brsv) Antibodies.pt_BR
dc.contributor.authorDomingues, Helena Gallicchiopt_BR
dc.contributor.authorCampalans, Jacquelinept_BR
dc.contributor.authorAlmeida, Renata Servanpt_BR
dc.contributor.authorCoswig, Lia Treptowpt_BR
dc.contributor.authorArns, Clarice Weispt_BR
unicamp.authorHelena Gallicchio Domingues, Departamento de Microbiologia e Imunologia, Instituto de Biologia, Universidade Estadual de Campinas-UNICAMP, SP, Brazil.pt_BR Campalans,pt Servan Almeida,pt Treptow Coswig,pt Weis Arns,pt
dc.subjectAntibodies, Viralpt_BR
dc.subjectAntigens, Viralpt_BR
dc.subjectCattle Diseasespt_BR
dc.subjectCells, Culturedpt_BR
dc.subjectEnzyme-linked Immunosorbent Assaypt_BR
dc.subjectNeutralization Testspt_BR
dc.subjectPredictive Value Of Testspt_BR
dc.subjectReagent Kits, Diagnosticpt_BR
dc.subjectRespiratory Syncytial Virus Infectionspt_BR
dc.subjectRespiratory Syncytial Virus, Bovinept_BR
dc.subjectSensitivity And Specificitypt_BR
dc.description.abstractA dot-ELISA test for the detection of anti-BRSV antibodies is described. The objective of this study was the standardisation of a test as a fast, inexpensive and effective alternative to detect anti-BRSV antibodies. Its sensitivity, specificity and usefulness were compared to a commercial ELISA-kit and to the standard serum neutralisation (SN) test. The standardisation of the technique was done using nitrocellulose disks soaked with a viral sample isolated in Brazil, BRSV-25-BR. The best results were obtained when the disks were sensitised with a purified antigen at a concentration of 0.7 microg/disk and the bovine serum was diluted 1: 200. The experiment used 423 samples of bovine serum collected in the main cattle breeding centres in Brazil. The standard SN, dot-ELISA technique and commercial ELISA kits scored 67.8%, 71.8% and 72.3% of the samples as positive, respectively. When compared to the SN test, the standardised dot-ELISA and the commercial ELISA tests presented relative sensitivities of 92.3% and 91.6% and relative specificities of 71.3% and 68.4% respectively. The results demonstrated that the dot-ELISA test is adequate for the objectives proposed by this study, being easy to use and economically viable. Thus, this test represents an alternative for BRSV serological diagnosis in the substitution of SN and commercial ELISA tests, recommendable for utilisation in laboratories with few resources.en
dc.relation.ispartofVeterinary Researchpt_BR
dc.relation.ispartofabbreviationVet. Res.pt_BR
dc.identifier.citationVeterinary Research. v. 33, n. 4, p. 397-404pt_BR
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