Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/195052
Type: Artigo de periódico
Title: Role Of Kinins And Sensory Neurons In The Rat Pleural Leukocyte Migration Induced By Phoneutria Nigriventer Spider Venom.
Author: Costa, Soraia K P
Moreno, Ronilson A
Esquisatto, Laura C M
Juliano, Luiz
Brain, Susan D
De Nucci, Gilberto
Antunes, Edson
Abstract: The leukocyte migration induced by Phoneutria nigriventer spider venom (PNV) has been investigated in rats using the pleurisy model. Intrapleural injection of PNV (10-100 microg/cavity) caused a dose- and time-dependent leukocyte accumulation. The bradykinin B(2) receptor antagonist Hoe 140 (0.5 mg/kg) substantially inhibited PNV-induced cell accumulation, whereas the angiotensin-converting enzyme inhibitor captopril (2 mg/kg) potentiated by 80% this effect. The non-specific kallikrein inhibitor aprotinin and the plasma kallikrein inhibitor soybean trypsin inhibitor greatly reduced PNV-induced leukocyte migration, whereas the selective tissue kallikrein inhibitor P(ac)-F-S-R-EDDnp failed to affect PNV-induced responses. Treatment of rats with capsaicin (50 mg/kg) at the neonatal stage resulted in 67% inhibition of the PNV-induced cell migration. The neurokinin NK(1) receptor antagonist SR140333, but not the NK(2) receptor antagonist SR48968, reduced by 55% venom-induced cell accumulation. We conclude that bradykinin generation is involved in the PNV-induced pleural leukocyte migration in rats, where it can directly activate sensory nerves contributing to a neurogenic inflammatory mechanism.
Subject: Angiotensin-converting Enzyme Inhibitors
Animals
Aprotinin
Benzamides
Bradykinin
Bradykinin Receptor Antagonists
Capsaicin
Chemotaxis, Leukocyte
Dose-response Relationship, Drug
Leukocytes
Male
Neurogenic Inflammation
Neurokinin-1 Receptor Antagonists
Neurons, Afferent
Piperidines
Pleura
Pleurisy
Quinuclidines
Rats
Rats, Wistar
Receptors, Bradykinin
Receptors, Neurokinin-1
Soybean Proteins
Spider Venoms
Trypsin Inhibitors
Citation: Neuroscience Letters. v. 318, n. 3, p. 158-62, 2002-Feb.
Rights: fechado
Address: http://www.ncbi.nlm.nih.gov/pubmed/11803123
Date Issue: 2002
Appears in Collections:Unicamp - Artigos e Outros Documentos

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