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dc.typeArtigo de periódicopt_BR
dc.titleThe Hemolytic Component Of Cancer Anemia: Effects Of Osmotic And Metabolic Stress On The Erythrocytes Of Rats Bearing Multifocal Inoculations Of The Walker 256 Tumor.pt_BR
dc.contributor.authorVido, A Apt_BR
dc.contributor.authorCavalcanti, T Cpt_BR
dc.contributor.authorGuimarães, Fpt_BR
dc.contributor.authorVieira-Matos, A Npt_BR
dc.contributor.authorRettori, Opt_BR
unicamp.authorA A Vido, Departamento de Bioquímica, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP.pt_BR C Cavalcanti,pt Guimarães,pt N Vieira-Matos,pt Rettori,pt
dc.subjectAnalysis Of Variancept_BR
dc.subjectAnemia, Hemolyticpt_BR
dc.subjectCarcinoma 256, Walkerpt_BR
dc.subjectChronic Diseasept_BR
dc.subjectDisease Models, Animalpt_BR
dc.subjectNeoplasm Transplantationpt_BR
dc.subjectOsmotic Fragilitypt_BR
dc.subjectRats, Wistarpt_BR
dc.subjectTime Factorspt_BR
dc.description.abstractCancer anemia is classified as an anemia of chronic diseases, although it is sometimes the first symptom of cancer. Cancer anemia includes a hemolytic component, important in the terminal stage when even transfused cells are rapidly destroyed. The presence of a chronic component and the terminal complications of the illness limit studies of the hemolytic component. A multifocal model of tumor growth was used here to simulate the terminal metastatic dissemination stage (several simultaneous inoculations of Walker 256 cells). The hemolytic component of anemia began 3-4 days after inoculation in 100% of the rats and progressed rapidly thereafter: Hb levels dropped from 14.9 +/- 0.02 to 8.7 +/- 0.06 from days 7 to 11 ( approximately 5 times the physiologically normal rate in rats) in the absence of bleeding. The development of anemia was correlated (r2 = 0.86) with the development of other systemic effects such as anorexia. There was a significant decrease in the osmotic fragility of circulating erythrocytes: the NaCl concentration causing 50% lysis was reduced from 4.52 +/- 0.06 to 4.10 +/- 0.01 (P<0.01) on day 7, indicating a reduction in erythrocyte volume. However, with mild metabolic stress (4-h incubation at 37oC), the erythrocytes showed a greater increase in osmotic fragility than the controls, suggesting marked alteration of erythrocyte homeostasis. These effects may be due to primary plasma membrane alterations (transport and/or permeability) and/or may be secondary to metabolic changes. This multifocal model is adequate for studying the hemolytic component of cancer anemia since it is rapid, highly reproducible and causes minimal animal suffering.en
dc.relation.ispartofBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]pt_BR
dc.relation.ispartofabbreviationBraz. J. Med. Biol. Res.pt_BR
dc.identifier.citationBrazilian Journal Of Medical And Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas / Sociedade Brasileira De Biofísica ... [et Al.]. v. 33, n. 7, p. 815-22, 2000-Jul.pt_BR
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