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Type: Artigo de periódico
Title: Spontaneous Erythroid Colony Formation In Brazilian Patients With Sickle Cell Disease.
Author: Perlingeiro, R C
Costa, F F
Saad, S T
Arruda, V R
Queiroz, M L
Abstract: The ability of circulating progenitor cells to develop erythroid colonies was studied in vitro in the presence or absence of growth factors (5637-CM and erythropoietin) in 63 patients with sickle cell disease (SCD) (36 homozygotes for hemoglobin [Hb] S, 13 double heterozygotes for Hb S and beta thalassemia, and 14 SC patients) in Southeast Brazil. In the presence of growth factors, SCD patients (all genotypes) presented significantly higher numbers of circulating burst-forming unit-erythroid (BFU-E/5 x 10(5) MNC), when compared with control subjects. However, when the progenitor cells were cultured in the absence of added stimulus, high numbers of BFU-E were observed only in the genotypes SS and S/beta thalassemia. SC patients presented a similar response to the control subjects. Moreover, there was an inverse correlation between spontaneous (without stimulus) BFU-E and Hb levels in SCD patients. These results suggest that the formation of spontaneous BFU-E observed in SCD may be due to an expanded erythropoiesis secondary to hemolysis.
Subject: Adult
Anemia, Sickle Cell
Cell Differentiation
Cell Division
Colony-forming Units Assay
Erythroid Precursor Cells
Hemoglobin Sc Disease
Recombinant Proteins
Sickle Cell Trait
Citation: American Journal Of Hematology. v. 61, n. 1, p. 40-5, 1999-May.
Rights: fechado
Date Issue: 1999
Appears in Collections:Unicamp - Artigos e Outros Documentos

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