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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleChanges In Calcium Uptake Rate By Rat Cardiac Mitochondria During Postnatal Development.pt_BR
dc.contributor.authorBassani, R Apt_BR
dc.contributor.authorFagian, M Mpt_BR
dc.contributor.authorBassani, J Wpt_BR
dc.contributor.authorVercesi, A Ept_BR
unicamp.authorR A Bassani, Centro de Engenharia Biomédica and Departmento de Engenharia Biomédica, Faculdade de Engenharia Elétrica e de Computação, Universidade Estadual de Campinas, 13083-970 Campinas, SP, Brazil.pt_BR
unicamp.author.externalM M Fagian,pt
unicamp.author.externalJ W Bassani,pt
unicamp.author.externalA E Vercesi,pt
dc.subjectAgingpt_BR
dc.subjectAnimalspt_BR
dc.subjectAnimals, Newbornpt_BR
dc.subjectCalciumpt_BR
dc.subjectCarbonyl Cyanide P-trifluoromethoxyphenylhydrazonept_BR
dc.subjectHeartpt_BR
dc.subjectIntracellular Membranespt_BR
dc.subjectKineticspt_BR
dc.subjectMembrane Potentialspt_BR
dc.subjectMitochondria, Heartpt_BR
dc.subjectOxygen Consumptionpt_BR
dc.subjectRatspt_BR
dc.subjectRats, Wistarpt_BR
dc.subjectRuthenium Redpt_BR
dc.subjectSuccinatespt_BR
dc.description.abstractCa2+ uptake, transmembrane electrical potential (Deltapsim) and oxygen consumption were measured in isolated ventricular mitochondria of rats from 3 days to 5 months of age. Estimated values of ruthenium red-sensitive, succinate-supported maximal rate of Ca2+ uptake (Vmax, expressed as nmol Ca2+/min/mg protein) were higher in neonates and gradually fell during postnatal development (from 435+/-24 at 3-6 days, to 156+/-10 in adults,P<0.001), whereas K0.5 values (approximately 10 microM were not significantly affected by age. Under similar conditions, mitochondria from adults (5 months old) and neonates (4-6 days old) showed comparable state 4 (succinate and alpha-ketoglutarate as substrates) and state 3ADP (alpha-ketoglutarate-supported) respiration rates, as well as Deltapsim values (approximately-150 mV). Respiration-independent Deltapsim and Ca2+ uptake, supported by valinomycin-induced K+ efflux were also investigated at these ages. A transient Deltapsim (approximately -30 mV) was evoked by valinomycin in both neonatal and adult mitochondria. Respiration-independent Ca2+ uptake was also transient, but its initial rate was significantly higher in neonates than in adults (49. 4+/-10.0v 28.0+/-5.7 mmol Ca2+/min/mg protein,P<0.01). These results indicate that Ca2+ uptake capacity of rat cardiac mitochondria is remarkably high just after birth and declines over the first weeks of postnatal life, without change in apparent affinity of the transporter. Increased mitochondrial Ca2+ uptake rate in neonates appears to be related to the uniporter itself, rather than to modification of the driving force of the transport.en
dc.relation.ispartofJournal Of Molecular And Cellular Cardiologypt_BR
dc.relation.ispartofabbreviationJ. Mol. Cell. Cardiol.pt_BR
dc.date.issued1998-Octpt_BR
dc.identifier.citationJournal Of Molecular And Cellular Cardiology. v. 30, n. 10, p. 2013-23, 1998-Oct.pt_BR
dc.language.isoengpt_BR
dc.description.volume30pt_BR
dc.description.firstpage2013-23pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright 1998 Academic Presspt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn0022-2828pt_BR
dc.identifier.doi10.1006/jmcc.1998.0762pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/9799655pt_BR
dc.date.available2015-11-27T12:19:23Z-
dc.date.accessioned2015-11-27T12:19:23Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T12:19:23Z (GMT). No. of bitstreams: 1 pmed_9799655.pdf: 205889 bytes, checksum: 0aaf2504523870e960c0c12b0a9bfe73 (MD5) Previous issue date: 1998en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/194254-
dc.identifier.idPubmed9799655pt_BR
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