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Type: Artigo de periódico
Title: Inhibitory Effect Of Dipyridamole And Its Derivatives On Lipid Peroxidation In Mitochondria.
Author: Nepomuceno, M F
Alonso, A
Pereira-da-Silva, L
Tabak, M
Abstract: Dipyridamole (DIP), 2,6-bis(diethanolamino)-4,8-dipiperidino-[5,4-d] pyrimidine, is a coronary vasodilator widely used in clinics. It has also been reported to have coactivator activity for a number of antitumour drugs and antioxidant activity in membrane systems. In recent years we have been studying the spectroscopic properties of this drug and several of its derivatives as well as their interaction with charged micelles and phospholipid monolayers. A strong interaction of DIP and DIP derivatives with these model membrane systems and a dependence of the strength of the interaction upon the chemical structure of the DIP derivative was observed. Here, the antioxidant effect of DIP and the derivatives, RA14, RA47, and RA25, was compared. We observed that although it strongly inhibits the iron-induced lipoperoxidation on mitochondria (IC50 = 1 microM), it shows no protection against an organic oxidant, cumene hydroperoxide. The order of hydrophobicity of the DIP derivatives, DIP > RA14 > RA47 > RA25, correlates very well with both the values of the association constants of these derivatives to micelles, their localization in the micelles, and phospholipid films and their antioxidant effect on mitochondria. So, a very good correlation of the structure of the drug in regarded to the nature of its substituents with the biological activity is observed. Essentially the same result was observed either measuring the lipid peroxidation or the membrane fluidity by ESR, suggesting that the effect of DIP and DIP derivatives is probably associated to their binding to the lipid bilayer and not to interaction with membrane proteins.
Subject: Animals
Electron Spin Resonance Spectroscopy
Lipid Peroxidation
Membrane Fluidity
Mitochondria, Liver
Mitochondrial Swelling
Molecular Structure
Rats, Wistar
Vasodilator Agents
Citation: Free Radical Biology & Medicine. v. 23, n. 7, p. 1046-54, 1997.
Rights: fechado
Date Issue: 1997
Appears in Collections:Unicamp - Artigos e Outros Documentos

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