Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/193976
Type: Artigo de periódico
Title: The Applicability Of Hematoxylin-eosin Staining Plus Fluorescence Or Confocal Laser Scanning Microscopy To The Study Of Elastic Fibers In Cartilages.
Author: de Carvalho, H F
Taboga, S R
Abstract: This study focuses on the use of hematoxylin-eosin staining plus fluorescence microscopy for the investigation of elastic fibers in some elastic cartilages. We have observed that elastic fibers are consistently imaged by the proposed procedure and the resolution attained is similar to that obtained with the classical Weigert's fuchsin-resorcin. The results also demonstrate that elastin autofluorescence gives little or no contribution to the final fluorescence and that the use of the confocal laser scanning microscope adds to the resolution, permits the use of thicker sections and reveals of minute structural at features. We conclude that this is a relevant tool in elastin research.
Subject: Animals
Cartilage
Dogs
Elastic Tissue
Eosine Yellowish-(ys)
Fluorescent Dyes
Hematoxylin
Microscopy, Confocal
Microscopy, Fluorescence
Resorcinols
Rosaniline Dyes
Citation: Comptes Rendus De L'académie Des Sciences. Série Iii, Sciences De La Vie. v. 319, n. 11, p. 991-6, 1996-Nov.
Rights: fechado
Address: http://www.ncbi.nlm.nih.gov/pubmed/9064122
Date Issue: 1996
Appears in Collections:Unicamp - Artigos e Outros Documentos

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