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Type: Artigo de periódico
Title: Oxidative Damage Of Mitochondria Induced By Fe(ii)citrate Is Potentiated By Ca2+ And Includes Lipid Peroxidation And Alterations In Membrane Proteins.
Author: Castilho, R F
Meinicke, A R
Almeida, A M
Hermes-Lima, M
Vercesi, A E
Abstract: Isolated rat liver mitochondria exposed to Fe(II)citrate undergo lipid peroxidation and alterations in membrane proteins. These processes were associated with irreversible decrease in membrane potential and mitochondrial swelling. Lipid peroxidation was evidenced by the production of thiobarbituric acid-reactive substances and also by the reaction of these products with membrane proteins, through the formation of Schiff bases. Alterations in membrane proteins were also characterized by the loss of specific proteins that could be recovered from the mitochondrial supernatant as shown by SDS-polyacrylamide gel electrophoresis. The degree of both lipid peroxidation and alterations in membrane proteins were diminished by EGTA, ruthenium red, or dibucaine. This strongly indicates that Ca2+ potentiates the oxidative damage of mitochondria exposed to Fe(II)citrate.
Subject: Animals
Drug Synergism
Egtazic Acid
Ferrous Compounds
Intracellular Membranes
Lipid Peroxidation
Membrane Potentials
Membrane Proteins
Mitochondria, Liver
Mitochondrial Swelling
Onium Compounds
Organophosphorus Compounds
Oxygen Consumption
Rats, Wistar
Ruthenium Red
Time Factors
Citation: Archives Of Biochemistry And Biophysics. v. 308, n. 1, p. 158-63, 1994-Jan.
Rights: fechado
Date Issue: 1994
Appears in Collections:Unicamp - Artigos e Outros Documentos

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