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Type: Artigo de periódico
Title: Image Analysis Of Feulgen-stained C-h-ras-transformed Nih/3t3 Cells.
Author: Mello, M L
Russo, J
Abstract: Feulgen-DNA content, nuclear phenotypes, and levels of chromatin condensation were evaluated by image analysis in NIH/3T3 cells transformed with the c-H-ras oncogene of T24 cells. Three nuclear phenotypes, differing from those of untransformed control cells and defined in terms of patterns of chromatin condensation, were demonstrated microspectrophotometrically for the tumor cells. Polyploidy could only be observed in nuclei with extensive and deeply stained areas covered with condensed chromatin, i.e., only in a small fraction of the tumor cell nuclear population. The increased chromatin condensation that appeared with cell transformation affected the euchromatin zones. The image analysis provided data that, compared with those obtained in other situations involving cell transformation, could be relevant to the understanding of changes in chromatin supraorganization related to tumorigenesis and to tumor cell diagnosis.
Subject: Animals
Cell Line, Transformed
Cell Nucleus
Cell Transformation, Neoplastic
Coloring Agents
Dna, Neoplasm
Genes, Ras
Image Processing, Computer-assisted
Mice, Nude
Neoplasm Transplantation
Rosaniline Dyes
Citation: Biochemistry And Cell Biology = Biochimie Et Biologie Cellulaire. v. 68, n. 7-8, p. 1026-31
Rights: fechado
Appears in Collections:Unicamp - Artigos e Outros Documentos

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