Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/104200
Type: Artigo
Title: Dna content and chromatin texture of human breast epithelial cells transformed with 17-β-estradiol and the estrogen antagonist ICI 182,780 as assessed by image analysis
Title Alternative: 
Author: Mello, Maria Luiza S.
Vidal, Benedicto C.
Russo, Irma H.
Lareef, Mohamed H.
Russo, Jose
Abstract: The immortalized human breast epithelial MCF-10F cell line, although estrogen receptor α negative, develops cell proliferating activities and invasiveness indicative of neoplastic transformation, after treatment with 17-β-estradiol (E-2). These effects are similar to those produced by benzo[a]pyrene (BP). Since we have previously reported changes in the nuclear parameters accompanying BP-induced tumorigenesis in MCF-10F cells, we have examined whether similar alterations occur in E-2-treated cells. We therefore studied DNA amounts and other nuclear parameters in Feulgen-stained MCF-10F cells after treatment with various concentrations of E-2, BP, the estrogen antagonist ICI 182,780, and E-2 in the presence of ICI 182,780. E-2 caused a certain loss of DNA and changes in the nuclear size and chromatin supraorganization of MCF-10F cells. Many of these changes were similar to those produced by BP and were indicative of neoplastic transformation. More intense chromatin remodelling was seen with 70 nM E-2. Since these changes were not abrogated totally or partially by ICI 182,780, the neoplastic transformation of MCF-10F cells stimulated by E-2 involved a process that was independent of estrogen α-receptors. The changes produced by ICI 182,780 alone were attributed to effects other than its well-known anti-estrogenic activity. © 2007 Elsevier B.V. All rights reserved.
The immortalized human breast epithelial MCF-10F cell line, although estrogen receptor α negative, develops cell proliferating activities and invasiveness indicative of neoplastic transformation, after treatment with 17-β-estradiol (E-2). These effects are similar to those produced by benzo[a]pyrene (BP). Since we have previously reported changes in the nuclear parameters accompanying BP-induced tumorigenesis in MCF-10F cells, we have examined whether similar alterations occur in E-2-treated cells. We therefore studied DNA amounts and other nuclear parameters in Feulgen-stained MCF-10F cells after treatment with various concentrations of E-2, BP, the estrogen antagonist ICI 182,780, and E-2 in the presence of ICI 182,780. E-2 caused a certain loss of DNA and changes in the nuclear size and chromatin supraorganization of MCF-10F cells. Many of these changes were similar to those produced by BP and were indicative of neoplastic transformation. More intense chromatin remodelling was seen with 70 nM E-2. Since these changes were not abrogated totally or partially by ICI 182,780, the neoplastic transformation of MCF-10F cells stimulated by E-2 involved a process that was independent of estrogen α-receptors. The changes produced by ICI 182,780 alone were attributed to effects other than its well-known anti-estrogenic activity.
The immortalized human breast epithelial MCF-10F cell line, although estrogen receptor α negative, develops cell proliferating activities and invasiveness indicative of neoplastic transformation, after treatment with 17-β-estradiol (E-2). These effects are similar to those produced by benzo[a]pyrene (BP). Since we have previously reported changes in the nuclear parameters accompanying BP-induced tumorigenesis in MCF-10F cells, we have examined whether similar alterations occur in E-2-treated cells. We therefore studied DNA amounts and other nuclear parameters in Feulgen-stained MCF-10F cells after treatment with various concentrations of E-2, BP, the estrogen antagonist ICI 182,780, and E-2 in the presence of ICI 182,780. E-2 caused a certain loss of DNA and changes in the nuclear size and chromatin supraorganization of MCF-10F cells. Many of these changes were similar to those produced by BP and were indicative of neoplastic transformation. More intense chromatin remodelling was seen with 70 nM E-2. Since these changes were not abrogated totally or partially by ICI 182,780, the neoplastic transformation of MCF-10F cells stimulated by E-2 involved a process that was independent of estrogen α-receptors. The changes produced by ICI 182,780 alone were attributed to effects other than its well-known anti-estrogenic activity.
Subject: Células epiteliais
Mamas - Câncer
Estradiol
Benzo(a)pireno
Ploidia
Cromatina
Country: Holanda
Editor: Elsevier
Citation: Mutation Research - Fundamental And Molecular Mechanisms Of Mutagenesis. , v. 617, n. 01/02/15, p. 1 - 7, 2007.
Rights: Fechado
Identifier DOI: 10.1016/j.mrfmmm.2006.08.013
Address: https://www.sciencedirect.com/science/article/pii/S0027510706003472
Date Issue: 2007
Appears in Collections:IB - Artigos e Outros Documentos

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